PXD039974

PXD039974 is an original dataset announced via ProteomeXchange.

Title	The study of developing human deciduous molar germ during cap to bell transition
Description	Tooth is a good model for studying stem cell driving epithelial and mesenchymal cells development and differentiation. Identifying key factors and pathways governing tooth development will help elucidate the physiological and pathological processes including genetic defects. To investigate the time-dependent proteomic changes of tooth development, label-free quantitative proteomics were conducted for human deciduous molars collected from post conception week (PCW) 12, 15 and 18, encompassing cap to early bell stage. The proteomic profiles reveal dynamic changes of driving proteins and canonical signaling pathways governing tooth development. The proteins with different expression patterns were identified and the protein-protein interaction networks were constructed. Besides, a series of proteins that were potential or novel drivers of tooth morphogenesis had been screened. The polarized expression patterns in odontogenic mesenchymal cell and muscle cell development and the bipolar expression pattern in odontogenic epithelial cell development were revealed. Three candidate proteins (GJA1, PAX9 and KRT15) with different expression patterns were validated by immunohistochemistry. Our study provides an invaluable resource for further mechanistic studies and suggests core factors regulating tooth development.
HostingRepository	iProX
AnnounceDate	2023-02-09
AnnouncementXML	Submission_2023-02-08_19:11:57.904.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaohang Chen
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-08 19:11:43	ID requested
⏵ 1	2023-02-08 19:11:58	announced

Dataset with its publication pending

submitter keyword: Human deciduous molar, label-free proteome, protein expression pattern, cap to bell stage

Fengxiang Wei
contact affiliation	Longgang Maternity and Child Healthcare Hospital
contact email	haowei727499@163.com
lab head
Xiaohang Chen
contact affiliation	Longgang Maternity and Child Healthcare Hospital
contact email	769778984@qq.com
dataset submitter

iProX dataset URI