PXD039935

PXD039935 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Data independent acquisition mass spectrometry of isolated glomeruli from mutant mice lacking the nuclear export signal of p62 and WT
Description	＜Project description＞ Data independent acquisition (DIA) mass spectrometry of Isolated glomeruli from homozygous deletion of the nuclear export signal of p62 (dNES) and WT mice (n=3 each). ＜Sample processing＞ Formalin fixed paraffin embedded (FFPE) tissues were prepared in general method and kidney samples were cut into 5µm-thick sections on the membrane slides (Leica Microsystems, Mannheim, Germany). Glomeruli were isolated via laser capture micro-dissection (0.6 mm2 in total). The dissected glomeruli were collected separately into 40 µL of Tris EDTA buffer and 0.002% Zwittergent 3-16 detergent.　Solubilised proteins were further digested into peptides by 0.01ug/ul trypsin (Promega, Madison, WI, USA) overnight. Each sample was centrifuged at 15000 rpm for 30 minutes prior to measurement. ＜Mass spectrometry and data processing＞ Samples were measured by DIA-MSMS using NanoLC-MSMS system with Vanquish Neo and Orbitrap Exploris 240 (Thermo Scientific). The measurement data were analyzed with DIA-NN 1.8.1.
HostingRepository	jPOST
AnnounceDate	2024-02-08
AnnouncementXML	Submission_2024-02-07_07:00:40.318.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kunio Kawanishi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	unknown modification
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-07 23:17:40	ID requested
⏵ 1	2024-02-07 07:00:40	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: p62, nuclear export signal, kidney, glomeruli, mouse

submitter keyword: p62, nuclear export signal, kidney, glomeruli, mouse

Contact List

Eiji Warabi
lab head
Kunio Kawanishi
contact affiliation	University of Tsukuba
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002023/