PXD039933

PXD039933 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A Suspension Trapping-based Sample Preparation Workflow for Sensitive Plant Phosphoproteomics
Description	Plant phosphoproteomics reveals a global view of phosphorylation-mediated signaling in plants, but it remains demanding a high-throughput method with detection sensitivity and quantification accuracy. In general, the indispensable protein precipitation step requiring for interferences removal limited the sensitivity and throughput in plant phosphoproteomics analysis. Though protein precipitation improves the sample purity, the multiple handling steps cause sample loss and process variability. Here we developed a suspension trapping (S-Trap)-based approach, termed tandem S-Trap-IMAC, by integrating a S-Trap micro column with a Fe-IMAC tip for plant phosphoproteomics. In comparing to precipitation-based approach, tandem S-Trap-IMAC method deepened the coverage of Arabidopsis phosphoproteome more than 30% with improved quantification accuracy and short sample processing time compared to the precipitation-based workflow. The tandem S-Trap-IMAC method was further applied to study the abscisic acid (ABA) signaling in Arabidopsis seedlings. Among a total of 24,055 identified phosphopeptides, several key phosphorylation sites on core ABA signaling components were quantified across four time points. The optimized workflow aids in high-throughput phosphoproteome profiling of low-input plant samples.
HostingRepository	jPOST
AnnounceDate	2023-08-28
AnnouncementXML	Submission_2023-08-28_01:02:07.860.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuan-Chih Hsu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-07 18:48:20	ID requested
⏵ 1	2023-08-28 01:02:08	announced

Publication List

Chen CW, Tsai CF, Lin MH, Lin SY, Hsu CC, Suspension Trapping-Based Sample Preparation Workflow for In-Depth Plant Phosphoproteomics. Anal Chem, 95(33):12232-12239(2023) [pubmed]

Chen CW, Tsai CF, Lin MH, Lin SY, Hsu CC, Suspension Trapping-Based Sample Preparation Workflow for In-Depth Plant Phosphoproteomics. Anal Chem, 95(33):12232-12239(2023) [pubmed]

Keyword List

submitter keyword: Plant phosphoproteomics, suspension trapping

submitter keyword: Plant phosphoproteomics, suspension trapping

Contact List

Chuan-Chih Hsu
lab head
Chuan-Chih Hsu
contact affiliation	Purdue University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002028/