PXD039923

PXD039923 is an original dataset announced via ProteomeXchange.

Title	Proteomic comparison of human milk, cow's milk and infant formula extracellular vesicles
Description	Milk and milk products such as infant formula (IF) play a fundamental role in serving the nutritional needs of the developing infant. Extracellular vesicles (EVs) in human (HM) and cow’s milk (CM) contain molecular cargo such as proteins and micro(mi)RNA that serve as functional messengers between cells and may be of importance to infant health. Here, we have developed a pipeline using advanced proteomics and transcriptomics to enable cross-species comparison of milk and IF EVs. EVs from HM, CM and IF were subjected to data-independent acquisition mass spectrometry and RNA-seq. Differentially abundant proteins (143) and miRNAs (514) (false discovery rate < 0.01) were identified in HM and CM EVs, and CM EV proteins and miRNAs were conserved in IF EVs (~20-90%). We foresee this work to be used in large scale studies to determine biologically relevant species-specific differences in milk EVs that could be leveraged to improve IF products.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:50:27.884.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natalie Turner
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-07 14:05:48	ID requested
1	2023-08-13 08:06:08	announced
⏵ 2	2023-11-14 06:50:28	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: milk

infant formula

extracellular vesicles

proteomics

SWATH

mass spectrometry

transcriptomics

Murray D. Mitchell
contact affiliation	Centre for Children's Health Research (CCHR) Queensland University of Technology 62 Graham Street South Brisbane, QLD 4101 AUSTRALIA
contact email	murray.mitchell@qut.edu.au
lab head
Natalie Turner
contact affiliation	Queensland University of Technology
contact email	natalie.turner@hdr.qut.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD039923

PRIDE project URI

• PRIDE
  1. PXD039923
     1. Label: PRIDE project
     2. Name: Proteomic comparison of human milk, cow's milk and infant formula extracellular vesicles