PXD039923 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic comparison of human milk, cow's milk and infant formula extracellular vesicles |
Description | Milk and milk products such as infant formula (IF) play a fundamental role in serving the nutritional needs of the developing infant. Extracellular vesicles (EVs) in human (HM) and cow’s milk (CM) contain molecular cargo such as proteins and micro(mi)RNA that serve as functional messengers between cells and may be of importance to infant health. Here, we have developed a pipeline using advanced proteomics and transcriptomics to enable cross-species comparison of milk and IF EVs. EVs from HM, CM and IF were subjected to data-independent acquisition mass spectrometry and RNA-seq. Differentially abundant proteins (143) and miRNAs (514) (false discovery rate < 0.01) were identified in HM and CM EVs, and CM EV proteins and miRNAs were conserved in IF EVs (~20-90%). We foresee this work to be used in large scale studies to determine biologically relevant species-specific differences in milk EVs that could be leveraged to improve IF products. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_06:50:27.884.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Natalie Turner |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-07 14:05:48 | ID requested | |
1 | 2023-08-13 08:06:08 | announced | |
⏵ 2 | 2023-11-14 06:50:28 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: milk |
infant formula |
extracellular vesicles |
proteomics |
SWATH |
mass spectrometry |
transcriptomics |
Contact List
Murray D. Mitchell |
contact affiliation | Centre for Children's Health Research (CCHR) Queensland University of Technology 62 Graham Street South Brisbane, QLD 4101 AUSTRALIA |
contact email | murray.mitchell@qut.edu.au |
lab head | |
Natalie Turner |
contact affiliation | Queensland University of Technology |
contact email | natalie.turner@hdr.qut.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD039923 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039923
- Label: PRIDE project
- Name: Proteomic comparison of human milk, cow's milk and infant formula extracellular vesicles