PXD039922 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SPARK-mNG-mAID Immunoprecipitation Mass Spectrometry |
Description | SPARK is an AGC kinase found in apicomplexan parasites. In previous work, we showed that SPARK knockdown leads to inefficient T. gondii calcium mobilization, egress, and invasion of host cells. We performed immunoprecipitation and mass spectrometry to determine the interaction partners of SPARK-mNG-mAID isolated from T. gondii lysates. We identified a strong interaction partner, which we term SPARKEL. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:49:39.645.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD039922 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alice Herneisen |
SpeciesList | scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601; |
ModificationList | methylthiolated residue; phosphorylated residue; acetylated residue; monohydroxylated residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-07 14:03:14 | ID requested | |
1 | 2024-10-08 11:20:19 | announced | |
⏵ 2 | 2024-10-22 06:49:40 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Toxoplasma gondii, AGC kinase, SPARK |
Contact List
Sebastian Lourido |
contact affiliation | Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology |
contact email | lourido@wi.mit.edu |
lab head | |
Alice Herneisen |
contact affiliation | Whitehead Institute for Biomedical Research, Cambridge, MA, USA 02142 Department of Biology, Massachusetts Insitute of Technology |
contact email | alice@wi.mit.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039922
- Label: PRIDE project
- Name: SPARK-mNG-mAID Immunoprecipitation Mass Spectrometry