PXD039916

PXD039916 is an original dataset announced via ProteomeXchange.

Title	Haemoproteomics of Bombus terrestris following pesticide exposure and interaction with Crithidia bombi
Description	Pesticides pose a potential threat to bee health, especially in combination with other stressors, like parasites. However, pesticide risk assessment tests them in isolation from other stresses. We tested acute oral doses of three pesticides - glyphosate, Amistar and sulfoxaflor - on the bumblebee, Bombus terrestris, alongside the gut parasite Crithidia bombi. We found no impact of any pesticide on parasite intensity and no impact of sulfoxaflor or glyphosate on survival or weight change. Amistar caused weight loss and 19-41% mortality. Haemoproteome analysis showed various protein dysregulations. The major pathways dysregulated are those involved in insect defences and immune responses, with Amistar having the strongest impact on these. Our results show that while no response can be seen at a whole organism level, MALDI BeeTyping® can detect effects. Mass spectrometry analysis of bee haemolymph represent a pertinent tool to evaluate the stressor impacts on bee health, even at the individual scale.
HostingRepository	PRIDE
AnnounceDate	2023-02-09
AnnouncementXML	Submission_2023-02-09_06:44:37.036.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	KarimArafah
SpeciesList	scientific name: Crithidia bombi; NCBI TaxID: 288676; scientific name: Bombus terrestris terrestris; NCBI TaxID: 1255232;
ModificationList	S-pyridylethyl-L-cysteine; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-07 13:44:39	ID requested
⏵ 1	2023-02-09 06:44:37	announced

Dataset with its publication pending

submitter keyword: Bumble bee

Immune response

MALDI BeeTyping

Differential proteomics

Dr. PhilippeBulet
contact affiliation	1-Institute for Advanced Biosciences, CR Inserm U1209, CNRS UMR5309 Université Grenoble Alpes ARN, Epigénétique et Stress / RNA, Epigenetics and Stress F-38000 Grenoble, France 2-Plateforme BioPark d'Archamps ArchParc, Bât. Le Forum 2 218 Avenue Marie Curie F-74160 Archamps, France
contact email	philippe.bulet@univ-grenoble-alpes.fr
lab head
KarimArafah
contact affiliation	Biopark Archamps
contact email	karim.arafah@biopark-archamps.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD039916

PRIDE project URI

• PRIDE
  1. PXD039916
     1. Label: PRIDE project
     2. Name: Haemoproteomics of Bombus terrestris following pesticide exposure and interaction with Crithidia bombi