PXD039886 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Distinct gain of function mechanism for the hot-spot mutant p53R175H |
Description | TP53 (p53) is the most commonly mutated gene in human cancers, and p53 missense mutations are present in more than 40% of all human tumors. Most p53 mutations are located within the DNA binding domain, including hotspot mutations R175H, R248W, and R273H. To elucidate the precise mechanism by which p53 missense mutants execute their gain-of-functions (GOFs) in vivo, we used a proteomic screen to identify any protein that recognizes the p53 DNA-binding domain (DBD) in a manner dependent on its mutation status. To do so, we first purified the potential binding proteins associated with the p53 DBD through multi-step affinity chromatography from a SFB-p53 H1299 stable cell line. The affinity-purified SFB-p53 interacting proteins were detected by liquid chromatography mass spectrometry/mass spectrometry (LC–MS/MS) and revealed a few cellular proteins that have been reported to interact with the DNA binding domain of p53, such as TP53BP1, USP28, and Sirt1. Interestingly, we also identified many new interacting proteins from the same complex. |
HostingRepository | PRIDE |
AnnounceDate | 2023-02-24 |
AnnouncementXML | Submission_2023-02-24_01:50:33.994.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD039886 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | huanli |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-06 05:45:18 | ID requested | |
1 | 2023-02-17 03:52:49 | announced | |
⏵ 2 | 2023-02-24 01:50:34 | announced | 2023-02-24: Updated project metadata. |
Publication List
Keyword List
submitter keyword: p53R175H, GOF, LSD2, ferroptosis, BACH1, oxidative stress, tumor suppression,p53, metastasis |
Contact List
ZhenyiSu |
contact affiliation | Columbia University Medical Center |
contact email | zs2391@cumc.columbia.edu |
lab head | |
huanli |
contact affiliation | COLUMBIA UNIVERSITY MEDICAL CENTER |
contact email | hl2977@cumc.columbia.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039886
- Label: PRIDE project
- Name: Distinct gain of function mechanism for the hot-spot mutant p53R175H