PXD039880

PXD039880 is an original dataset announced via ProteomeXchange.

Title	Discovery and characterization of a chemical probe targeting the zinc-finger ubiquitin-binding domain of HDAC6
Description	Cellular selectivity of a chemical probe for the UBD of HDAC6. A biotinylated analog of HDAC6 chemical probe SGC-UBD253 bound to streptavidin beads was used to enrich HDAC6 from cytoplasmic fraction of HEK293 lysate. A triplicate of each condition - competition with probe, control, DMSO - was done.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:07:53.738.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Suzanne Ackloo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-06 02:59:21	ID requested
1	2023-10-24 11:46:00	announced
⏵ 2	2023-11-14 09:07:55	announced	2023-11-14: Updated project metadata.

Harding RJ, Franzoni I, Mann MK, Szewczyk MM, Mirabi B, Ferreira de Freitas R, Owens DDG, Ackloo S, Scheremetjew A, Juarez-Ornelas KA, Sanichar R, Baker RJ, Dank C, Brown PJ, Barsyte-Lovejoy D, Santhakumar V, Schapira M, Lautens M, Arrowsmith CH, Discovery and Characterization of a Chemical Probe Targeting the Zinc-Finger Ubiquitin-Binding Domain of HDAC6. J Med Chem, 66(15):10273-10288(2023) [pubmed]

submitter keyword: ubiquitin binding domain, chemical probe, UBD, chemoproteomics,HDAC6

Cheryl H Arrowsmith
contact affiliation	CSO, Structural Genomics Consortium Professor, Medical Biophysics, University of Toronto Sr. Scientist, Princess Margaret Cancer Centre
contact email	Cheryl.Arrowsmith@uhnresearch.ca
lab head
Suzanne Ackloo
contact affiliation	University of Toronto
contact email	suzanne.ackloo@utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD039880

PRIDE project URI

• PRIDE
  1. PXD039880
     1. Label: PRIDE project
     2. Name: Discovery and characterization of a chemical probe targeting the zinc-finger ubiquitin-binding domain of HDAC6