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PXD039877

PXD039877 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphorylation of RADICAL-INDUCED CELL DEATH1 by Photoregulatory Protein Kinases
DescriptionRADICAL-INDUCED CELL DEATH1 (RCD1) is a multidomain protein with intrinsically disordered regions (IDRs) separating its highly structured domains. RCD1 regulates plant development and stress tolerance by interacting with numerous transcription factors (TFs) through its C-terminal RST domain. It has been shown to interact in a non-RST-dependent manner with Photoregulatory Protein Kinases (PPKs). In Arabidopsis, this recently discovered family of protein kinases is comprised of four members that localize to nuclear bodies (NBs), subnuclear non-membrane bound complexes of mostly unknown function. PPK-dependent phosphorylation has been shown to target proteins for degradation, thereby playing an essential role in protein turnover. Mass spectrometric analysis of the in vitro phosphorylated GST-RCD1 revealed several PPK-dependent RCD1 phosphopeptides that were also identified in the in vivo pull-down experiments. Most of these phosphosites were clustered in the IDR2, the intrinsically disordered region between the WWE and PARP-like domains. Using transgenic lines expressing non-phosphorylatable version of RCD1 we showed that phosphorylation of the IDR2 is involved in the regulation of the RCD1 affecting its subnuclear distribution to NBs of different size and number. Moreover, phosphorylation of IDR2 facilitates degradation of RCD1 and possibly also its partner TFs. Thus, it is necessary for the function of RCD1 as a negative transcriptional co-regulator.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:45:06.827.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJulia Vainonen
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-06 02:58:07ID requested
12023-04-21 00:57:22announced
22023-11-14 08:45:07announced2023-11-14: Updated project metadata.
Publication List
Vainonen JP, Gossens R, Krasensky-Wrzaczek J, De Masi R, Danciu I, Puukko T, Battchikova N, Jonak C, Wirthmueller L, Wrzaczek M, Shapiguzov A, Kangasj, ä, rvi J, Poly(ADP-ribose)-binding protein RCD1 is a plant PARylation reader regulated by Photoregulatory Protein Kinases. Commun Biol, 6(1):429(2023) [pubmed]
Keyword List
submitter keyword: PPK,Arabidopsis, RCD1, phosphorylation
Contact List
Jaakko Kangasjärvi
contact affiliationOrganismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, and Viikki Plant Science Center, University of Helsinki, Helsinki, Finland
contact emailjaakko.kangasjarvi@helsinki.fi
lab head
Julia Vainonen
contact affiliationUniversity of Helsinki
contact emailjulia.vainonen@helsinki.fi
dataset submitter
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