PXD039869

PXD039869 is an original dataset announced via ProteomeXchange.

Title	Suppression of mosquito Aedes aegypti population using a CRISPR-targeted leucine aminopeptidase 1 mutation
Description	Aedes aegypti are vectors of several devastating arboviruses infecting hundreds of millions of people annually. Controlling mosquito populations by regulating their reproduction is important to minimize viral transmission in the absence of effective antiviral therapies or vaccines. Here, we demonstrate that leucine aminopeptidase1 (LAP1), screened from SWATH-MS-based proteomic data of female spermathecae, is a crucial determinant in mosquito population expansion. Mitochondrial defects and aberrant autophagy of sperm in LAP1 mutant males (LAP1-/-), prepared using CRISPR-Cas9 system, resulted in a reduction of reproduction in wild-type females that mated with them. Additionally, we found that the fitness of LAP1-/- males was strong enough to efficiently transmit genetic changes to mosquito populations through a low number of hatchable offspring, making it to be a promising opportunity to suppress mosquito populations using LAP1-/- males. Importantly, we provide a novel target gene for genetic drive, further amplifying the function of LAP1 in reducing mosquito populations.
HostingRepository	iProX
AnnounceDate	2023-02-05
AnnouncementXML	Submission_2024-02-22_19:52:39.250.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaomei Sun
SpeciesList	scientific name: Aedes aegypti; NCBI TaxID: 7159;
ModificationList	iodoacetamide derivatized aspartic acid; methionine oxidation with neutral loss of 64 Da; acetylated residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-05 19:19:14	ID requested
⏵ 1	2024-02-22 19:52:40	announced

Sun X, Wang X, Shi K, Lyu X, Sun J, Raikhel AS, Zou Z, Leucine aminopeptidase1 controls egg deposition and hatchability in male Aedes aegypti mosquitoes. Nat Commun, 15(1):106(2024) [pubmed]

submitter keyword: Aedes aegypti, leucine aminopeptidase 1, spermathecae, autophagy, mitochondria, reproduction

Zhen Zou
contact affiliation	Institute of Zoology, Chinese Academy of Sciences
contact email	zouzhen@ioz.ac.cn
lab head
Xiaomei Sun
contact affiliation	Institute of Zoology, Chinese Academy of Sciencesy
contact email	sunxiaomei@ioz.ac.cn
dataset submitter

iProX dataset URI