PXD039863

PXD039863 is an original dataset announced via ProteomeXchange.

Title	Combined transcriptome and proteome analysis of the protein composition of the brochosomes of the leafhopper Nephotettix cincticeps
Description	Brochosomes are a unique coating on the integuments of Cicadellidae produced in specialized glandular sections of Malpighian tubules. However, knowledge of the protein composition of brochosome is very limited. In this study, transcriptomic and proteomic profiling was performed to characterize the protein composition of the brochosome in rice green leafhopper Nephotettix cincticeps. Brochosomes were collected from the forewings of leafhoppers using ultrasonic treatment allowing for more effective brochosome collection and shock treatment resulting in purer brochosomes . After combined proteomic and transcriptomic analysis, 12 putative major structural proteins of the brochosome (BSM) and 10 brochosome-associated proteins (BSAP) were identified. Each BSM has specific conserved motifs containing several cysteines cross-linked by disulfide bonds. BSAP, which may be loosely linked to the outer and inner surfaces of the brochosomes, might play an important role in the proper folding of the BSM and resisting pathogens. Apart from a few BSAPs associated with cyclase, homologs of BSM and BSAP were present exclusively in the insect family Cicadellidae rather than other Hemiptera species, suggesting that these proteins might be encoded by an orphan gene family specific to the Cicadellidae. This is the first study to identify and characterize the protein composition of the brochosome of N. cincticeps, which will provide a solid foundation for further studies of brochosome synthesis, function, and evolutionary origins.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_07:15:46.202.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	wei wu
SpeciesList	scientific name: Nephotettix cincticeps (Green rice leafhopper) (Selenocephalus cincticeps); NCBI TaxID: 94400;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-04 13:55:39	ID requested
⏵ 1	2024-01-26 07:15:47	announced

Wu W, Ye Z, Mao Q, Shan HW, Li JM, Chen JP, . Insects, 14(10):(2023) [pubmed]

10.3390/insects14100784;

submitter keyword: Brochosome, Nephotettix cincticeps, Leafhopper, Transcriptome, LC-MS/MS

wei wu
contact affiliation	State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China
contact email	wuwei1@nbu.edu.cn
lab head
wei wu
contact affiliation	+860 187 5016 2795
contact email	wuwei1@nbu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039863
     1. Label: PRIDE project
     2. Name: Combined transcriptome and proteome analysis of the protein composition of the brochosomes of the leafhopper Nephotettix cincticeps