PXD039852

PXD039852 is an original dataset announced via ProteomeXchange.

Title	Persulfidation of plant and bacteroid proteins is involved in legume nodule development and senescence
Description	Legumes establish symbiosis with soil rhizobia forming root nodules that fix atmospheric nitrogen. The central role of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in nodule biology has been clearly established. Recently, hydrogen sulfide (H2S) and other reactive sulfur species (RSS) have emerged as novel signaling molecules in animals and plants. A major mechanism by which ROS, RNS, and RSS fulfil their signaling role is the post-translational modification of proteins. To identify possible functions of H2S in nodule development and senescence, we used the tag-switch method to analyze quantitative changes in the persulfidation profile of common bean (Phaseolus vulgaris) nodules at different developmental stages. The proteomic analysis suggests that persulfidation plays a major regulatory role in plant and bacteroid metabolism and senescence. In addition, the effect of a H2S donor on several proteins involved in ROS and RNS homeostasis was investigated. The results obtained using nodule extracts and recombinant proteins suggest a crosstalk between H2S, ROS, and RNS, and a protective function of persulfidation on redox-sensitive enzymes from oxidative modifications that may cause enzyme inactivation. It is concluded that the general decrease of persulfidation levels observed in plant proteins of aging nodules is one of the mechanisms that cause the disruption of redox homeostasis leading to senescence.
HostingRepository	PRIDE
AnnounceDate	2024-06-16
AnnouncementXML	Submission_2024-06-15_23:08:23.326.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luis C. Romero
SpeciesList	scientific name: Phaseolus vulgaris (Kidney bean) (French bean); NCBI TaxID: 3885;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-03 15:30:03	ID requested
⏵ 1	2024-06-15 23:08:24	announced

10.1093/jxb/erad436;

Matamoros MA, Romero LC, Tian T, Rom, á, n Á, Duanmu D, Becana M, Persulfidation of plant and bacteroid proteins is involved in legume nodule development and senescence. J Exp Bot, 75(10):3009-3025(2024) [pubmed]

submitter keyword: Legumes, nodule development, post-translational modifications, symbiosis, persulfidation, nodule senescence, redox regulation

Luis C Romero
contact affiliation	Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC y Universidad de Sevilla, 41092 Sevilla, Spain
contact email	lromero@ibvf.csic.es
lab head
Luis C. Romero
contact affiliation	Instituto Bioquimica Vegetal y Fotosintesis-CSIC
contact email	lromero@ibvf.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD039852

PRIDE project URI

• PRIDE
  1. PXD039852
     1. Label: PRIDE project
     2. Name: Persulfidation of plant and bacteroid proteins is involved in legume nodule development and senescence