PXD039851

PXD039851 is an original dataset announced via ProteomeXchange.

Title	Substances in the mandibular glands mediate queen manipulation of larval development and colony transition from growth to reproduction in an annual social bumble bee
Description	Social organization is commonly dynamic with extreme examples in annual eusocial insects ("annual superorganisms"), but the signals and mechanisms regulating social organization remained elusive. In annual bumble bee colonies, larvae with a close contact to a queen do not differentiate into gynes, pupate at an earlier age, and are commonly smaller than siblings that do not contact a queen. We combined detailed observations, proteomics, microRNA transcriptomics, and gland removal surgery, to study the regulation of brood development and division of labor in the model bumble bee Bombus terrestris. We found that regurgitates fed to larvae by queens and workers differ in their protein and microRNA composition. The proteome of the regurgitate overlaps significantly with that of the mandibular (MG) and hypopharyngeal glands (HPG), suggesting that these exocrine glands are the sources of some regurgitate proteins. The MG and HPG proteomes , but not that of the salivary glands, differed between queens and workers, with the caste-specificity preserved for the MG and regurgitate proteomes. Queens subjected to a surgical removal of the MG showed normal behavior and brood care, but failed to manipulate the developmental program of the brood they reared. These findings suggest that substances in the queen MG are fed to larvae and influence their developmental program. As the colony grows, an increasing number of workers feed larvae and by that reduce the effects of the queen substances, such that she can no longer manipulate the development of all larvae, and the colony switches from producing workers (ergonomic phase) to gynes (reproductive phase).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:05:01.764.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rosi Fassler
SpeciesList	scientific name: Bombus terrestris terrestris; NCBI TaxID: 1255232;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-03 15:29:35	ID requested
1	2023-10-05 07:32:31	announced
2	2023-11-14 07:54:33	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:05:02	announced	2024-10-22: Updated project metadata.

10.1073/PNAS.2302071120;

submitter keyword: queen, bee, cephalic glands, worker,Bombus terrestris, proteome

Prof. Dana Reichmann
contact affiliation	Associate Professor, Vice president of the ISBMB society Dept. of Biological Chemistry, room 1-637 The Alexander Silberman Institute of Life Science The Hebrew University of Jerusalem Givat Ram Campus, Jerusalem, 91904, Israel
contact email	danare@mail.huji.ac.il
lab head
Rosi Fassler
contact affiliation	Hebrew University of Jerusalem
contact email	rosi.fassler@mail.huji.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039851
     1. Label: PRIDE project
     2. Name: Substances in the mandibular glands mediate queen manipulation of larval development and colony transition from growth to reproduction in an annual social bumble bee