PXD039839

PXD039839 is an original dataset announced via ProteomeXchange.

Title	Biochemical characterisation of Mer3 helicase interactions and the protection of meiotic recombination intermediates
Description	Crossing over between homologs is critical for the stable segregation of chromosomes during the first meiotic division. S. cerevisiae Mer3 (HFM1 in mammals) is a SF2 helicase and member of the ZMM group of proteins, that facilitates the formation of the majority of crossovers during meiosis. Here we describe the structural organisation of Mer3 and using AlphaFold modelling and XL-MS we further characterise the previously described interaction with Mlh1-Mlh2. We find that Mer3 also forms a previously undescribed complex with the recombination regulating factors Top3 and Rmi1 and that this interaction is competitive with Sgs1BLM helicase in a phospho-dependent manner. Using in vitro reconstituted D-loop assays we show that Mer3 inhibits the anti-recombination activity of Sgs1 helicase, but only in the presence of Dmc1. Thus we provide a mechanism whereby Mer3 interacts with a network of proteins to protect Dmc1 derived D-loops from dissolution.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:03:29.904.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tanja Bange
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-03 09:42:43	ID requested
1	2023-07-20 11:38:41	announced
⏵ 2	2023-11-14 09:03:30	announced	2023-11-14: Updated project metadata.

Altmannova V, Firlej M, M, ü, ller F, Janning P, Rauleder R, Rousova D, Sch, ä, ffler A, Bange T, Weir JR, Biochemical characterisation of Mer3 helicase interactions and the protection of meiotic recombination intermediates. Nucleic Acids Res, 51(9):4363-4384(2023) [pubmed]

submitter keyword: crossover, yeast, phospho proteomics,Meiosis, Mer3

John R. Weir
contact affiliation	Friedrich Miescher Laboratory of the Max Planck Society, Max-Planck-Ring 9, 72076 Tübingen, Germany.
contact email	john.weir@tuebingen.mpg.de
lab head
Tanja Bange
contact affiliation	LMU Munich
contact email	tanja.bange@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD039839

PRIDE project URI

• PRIDE
  1. PXD039839
     1. Label: PRIDE project
     2. Name: Biochemical characterisation of Mer3 helicase interactions and the protection of meiotic recombination intermediates