PXD039833

PXD039833 is an original dataset announced via ProteomeXchange.

Title	The proteome of large or small extracellular vesicles in pig seminal plasma differs and defines sources and biological functions. Spectral Library
Description	Seminal plasma contains many and morphologically heterogeneous extracellular vesicles (sEVs) which, released by cells of the testis, epididymis and accessory sex glands, are involved in male and female reproductive processes. The goal of this study was to describe the proteome of porcine sEVs isolated by a size exclusion chromatography (SEC)-based procedure and size-classed as small (S-EVs) or large (L-EVs). The sEV-subsets were characterized for their total protein concentration, morphology, size distribution, EV-specific protein markers and purity. A total of 988 proteins were identified by LC-MS/MS and 737 of them were quantified by SWATH in S-EVs, L-EVs and EVs-free samples. The differential expression analysis revealed 197 differentially abundant proteins between both EV-subsets, and 37 and 199 between S-EVs and L-EVs vs EVs-free samples, respectively. The gene ontology (GO) enrichment analysis of differentially abundant proteins suggested, by the type of protein detected, that S-EVs would be released mainly through an apocrine blebbing pathway and be involved in modulating the immune environment of the female reproductive tract as well as during sperm-oocyte interaction. In contrast, the protein types detected might imply L-EVs are released by fusion of multivesicular bodies with the plasma membrane and would be involved in sperm physiological processes, such as capacitation and avoidance of oxidative stress. In conclusion, this study provides a procedure capable of isolating of subsets of EVs from pig seminal plasma with a high degree of purity and shows differences in the proteomic profile between EV-subsets, suggesting different sources and biological functions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:00:14.599.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-03 06:05:52	ID requested
1	2023-02-15 00:06:51	announced
⏵ 2	2023-11-14 09:00:14	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: seminal plasma, proteomics, subsets, extracellular vesicles

Jordi Roca
contact affiliation	Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Spain
contact email	roca@um.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD039833

PRIDE project URI

• PRIDE
  1. PXD039833
     1. Label: PRIDE project
     2. Name: The proteome of large or small extracellular vesicles in pig seminal plasma differs and defines sources and biological functions. Spectral Library