PXD039829

PXD039829 is an original dataset announced via ProteomeXchange.

Title	Urinary Glycosylated IGF2 peptides in association with CKD
Description	Chronic kidney disease (CKD) is prevalent in 10% of world’s adult population, with Estimated Glomerular filtration rate (eGFR) and albuminuria employed in diagnosis. Role of protein glycosylation in causal mechanisms of CKD progression is largely unknown. Aim of this study was to identify urinary O-linked glycopeptides in association to CKD for better characterization of CKD molecular manifestations. Urine samples from 2 healthy and 8 CKD subjects were analyzed by CE-MS/MS and glycopeptide analysis using Proteome Discoverer 1.4. Distribution of identi-fied glycopeptides and correlation with Age, eGFR and Albuminuria were evaluated in 3810 da-tasets. In total, 17 O-linked glycopeptides from 7 different proteins were identified, derived primarily from Insulin-like growth factor-II (IGF2). Glycosylation occurred at the surface ex-posed IGF2 Threonine 96 position. Three glycopeptides (DVStPPTVLPDNFPRYPVGKF, DVStPPTVLPDNFPRYPVG and DVStPPTVLPDNFPRYP) exhibited positive correlation with Age. Glycopeptide (tPPTVLPDNFPRYP) showed strong negative association with eGFR. These results suggest that with aging and deteriorating kidney function, alterations in IGF2 proteoforms take place; which may reflect changes in mature IGF2 protein. Our results corroborate this hypothesis as IGF2 increased plasma levels were observed in CKD patients. Protease predictions, consider-ing also available transcriptomics data, suggest activation of cathepsin S with CKD, meriting further investigation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:26.156.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039829
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sonnal Lohia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	complex glycosylation
Instrument	Thermo Finnigan instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-03 06:02:50	ID requested
1	2023-05-10 14:20:03	announced
⏵ 2	2023-11-14 08:47:27	announced	2023-11-14: Updated project metadata.

Lohia S, Latosinska A, Zoidakis J, Makridakis M, Mischak H, Glorieux G, Vlahou A, Jankowski V, Glycosylation Analysis of Urinary Peptidome Highlights IGF2 Glycopeptides in Association with CKD. Int J Mol Sci, 24(6):(2023) [pubmed]

10.6019/PXD039829;

submitter keyword: CKD

eGFR

post translational modifications

PTMs

glycosylation

urine

peptidomics

glyco-peptide

IGF2

Antonia Vlahou
contact affiliation	Staff Research Scientist - Professor Level Center of Systems Biology Proteomics Laboratory Biomedical Research Foundation, Academy of Athens
contact email	vlahoua@bioacademy.gr
lab head
Sonnal Lohia
contact affiliation	Biomedical Research Foundation, Academy of Athens
contact email	sonnal08@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD039829

PRIDE project URI

• PRIDE
  1. PXD039829
     1. Label: PRIDE project
     2. Name: Urinary Glycosylated IGF2 peptides in association with CKD