PXD039824 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | New insight into the composition of extracellular traps released by macrophages exposed to different types of inducers |
Description | Neutrophil extracellular trap (NET) release plays a key role in many chronic disease settings, including atherosclerosis. They are critical to innate immune defence, but also contribute to disease by promoting thrombosis and inflammation. Macrophages also release extracellular traps or “METs”, but their composition and role in pathological processes are less well defined. In this study, we examined MET release from human THP-1 macrophages exposed to inflammatory and pathogenic stimuli, including tumour necrosis factor alpha (TNFalpha), hypochlorous acid (HOCl) and nigericin. In each case, there was release of DNA from the macrophages, as visualised by fluorescence microscopy with the cell impermeable DNA binding dye SYTOX green, consistent with MET formation. Proteomic analysis on METs released from macrophages exposed to TNFalpha and nigericin reveals that they are composed of linker and core histones, together with a range of cytosolic and mitochondrial proteins. These include proteins involved in DNA binding, stress responses, cytoskeletal organisation, metabolism, inflammation, anti-microbial activity and calcium binding. Quinone oxidoreductase in particular, was highly abundant in all METs but has not been reported previously in NETs. Moreover, there was an absence of proteases in METs in contrast to NETs. Some of the MET histones, contained post-translational modifications, including acetylation and methylation of Lys but not citrullination of Arg. These data provide new insight into the potential implications of MET formation in vivo and their contributions to immune defence and pathology. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:48:16.389.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Per Hägglund |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monomethylated residue; acetylated residue |
Instrument | Bruker Daltonics instrument model |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-03 03:09:56 | ID requested | |
1 | 2023-05-16 02:46:04 | announced | |
⏵ 2 | 2023-11-14 08:48:25 | announced | 2023-11-14: Updated project metadata. |
Publication List
Jensen M, Thorsen NW, Hallberg LAE, H, ä, gglund P, Hawkins CL, New insight into the composition of extracellular traps released by macrophages exposed to different types of inducers. Free Radic Biol Med, 202():97-109(2023) [pubmed] |
Keyword List
submitter keyword: post-translational modifications, histones, macrophages,Extracellular traps |
Contact List
Per Hägglund |
contact affiliation | University of Copenhagen Faculty of Health and Medical Sciences Department of Biomedical Sciences |
contact email | hagglundperm@gmail.com |
lab head | |
Per Hägglund |
contact affiliation | University of Copenhagen |
contact email | hagglundperm@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD039824 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039824
- Label: PRIDE project
- Name: New insight into the composition of extracellular traps released by macrophages exposed to different types of inducers