PXD039824

PXD039824 is an original dataset announced via ProteomeXchange.

Title	New insight into the composition of extracellular traps released by macrophages exposed to different types of inducers
Description	Neutrophil extracellular trap (NET) release plays a key role in many chronic disease settings, including atherosclerosis. They are critical to innate immune defence, but also contribute to disease by promoting thrombosis and inflammation. Macrophages also release extracellular traps or “METs”, but their composition and role in pathological processes are less well defined. In this study, we examined MET release from human THP-1 macrophages exposed to inflammatory and pathogenic stimuli, including tumour necrosis factor alpha (TNFalpha), hypochlorous acid (HOCl) and nigericin. In each case, there was release of DNA from the macrophages, as visualised by fluorescence microscopy with the cell impermeable DNA binding dye SYTOX green, consistent with MET formation. Proteomic analysis on METs released from macrophages exposed to TNFalpha and nigericin reveals that they are composed of linker and core histones, together with a range of cytosolic and mitochondrial proteins. These include proteins involved in DNA binding, stress responses, cytoskeletal organisation, metabolism, inflammation, anti-microbial activity and calcium binding. Quinone oxidoreductase in particular, was highly abundant in all METs but has not been reported previously in NETs. Moreover, there was an absence of proteases in METs in contrast to NETs. Some of the MET histones, contained post-translational modifications, including acetylation and methylation of Lys but not citrullination of Arg. These data provide new insight into the potential implications of MET formation in vivo and their contributions to immune defence and pathology.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:16.389.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Per Hägglund
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; acetylated residue
Instrument	Bruker Daltonics instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-03 03:09:56	ID requested
1	2023-05-16 02:46:04	announced
⏵ 2	2023-11-14 08:48:25	announced	2023-11-14: Updated project metadata.

Jensen M, Thorsen NW, Hallberg LAE, H, ä, gglund P, Hawkins CL, New insight into the composition of extracellular traps released by macrophages exposed to different types of inducers. Free Radic Biol Med, 202():97-109(2023) [pubmed]

submitter keyword: post-translational modifications, histones, macrophages,Extracellular traps

Per Hägglund
contact affiliation	University of Copenhagen Faculty of Health and Medical Sciences Department of Biomedical Sciences
contact email	hagglundperm@gmail.com
lab head
Per Hägglund
contact affiliation	University of Copenhagen
contact email	hagglundperm@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039824
     1. Label: PRIDE project
     2. Name: New insight into the composition of extracellular traps released by macrophages exposed to different types of inducers