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PXD039824

PXD039824 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNew insight into the composition of extracellular traps released by macrophages exposed to different types of inducers
DescriptionNeutrophil extracellular trap (NET) release plays a key role in many chronic disease settings, including atherosclerosis. They are critical to innate immune defence, but also contribute to disease by promoting thrombosis and inflammation. Macrophages also release extracellular traps or “METs”, but their composition and role in pathological processes are less well defined. In this study, we examined MET release from human THP-1 macrophages exposed to inflammatory and pathogenic stimuli, including tumour necrosis factor alpha (TNFalpha), hypochlorous acid (HOCl) and nigericin. In each case, there was release of DNA from the macrophages, as visualised by fluorescence microscopy with the cell impermeable DNA binding dye SYTOX green, consistent with MET formation. Proteomic analysis on METs released from macrophages exposed to TNFalpha and nigericin reveals that they are composed of linker and core histones, together with a range of cytosolic and mitochondrial proteins. These include proteins involved in DNA binding, stress responses, cytoskeletal organisation, metabolism, inflammation, anti-microbial activity and calcium binding. Quinone oxidoreductase in particular, was highly abundant in all METs but has not been reported previously in NETs. Moreover, there was an absence of proteases in METs in contrast to NETs. Some of the MET histones, contained post-translational modifications, including acetylation and methylation of Lys but not citrullination of Arg. These data provide new insight into the potential implications of MET formation in vivo and their contributions to immune defence and pathology.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:48:16.389.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPer Hägglund
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonomethylated residue; acetylated residue
InstrumentBruker Daltonics instrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-03 03:09:56ID requested
12023-05-16 02:46:04announced
22023-11-14 08:48:25announced2023-11-14: Updated project metadata.
Publication List
Jensen M, Thorsen NW, Hallberg LAE, H, ä, gglund P, Hawkins CL, New insight into the composition of extracellular traps released by macrophages exposed to different types of inducers. Free Radic Biol Med, 202():97-109(2023) [pubmed]
Keyword List
submitter keyword: post-translational modifications, histones, macrophages,Extracellular traps
Contact List
Per Hägglund
contact affiliationUniversity of Copenhagen Faculty of Health and Medical Sciences Department of Biomedical Sciences
contact emailhagglundperm@gmail.com
lab head
Per Hägglund
contact affiliationUniversity of Copenhagen
contact emailhagglundperm@gmail.com
dataset submitter
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Dataset FTP location
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