PXD039817

PXD039817 is an original dataset announced via ProteomeXchange.

Title	CREB regulated transcriptional coactivator 2 proteome landscape is plausibly modulated by SREBF1
Description	CRTC2 is a critical transcription cofactor that induces the glucose homeostatic genes by activating CREB. However, energy homeostasis is maintained by multiple pathways, therefore, it is possible that CRTC2 may interact with other transcription factors, especially under metabolic stress. Thereby, CRTC2 liver-specific knockout mice were created and the global proteome, phosphoproteome and acetylome from liver tissue under the high fat diet conditions were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and bioinformatics analysis. As expected, differentially expressed proteins (DEPs) are enriched in metabolic pathways that were subsequently corroborated by animal experiments. The consensus DEPs from these datasets were used as seed proteins to generate a protein-protein interaction (PPI) network using STRING and GeneMANIA identified fatty acid synthase (FASN) as the mutually relevant protein. Additional local-PPI (LPPI) analysis of CRTC2 and FASN with DEPs, SREBF1 was found to be a common mediator. CRTC2/CREB and SREBF1 are transcription factors, DNA-binding motif analysis showed multiple CRTC2/CREB regulated genes also possess SREBF1 binding motifs that unveil the possible induction by CRTC2/SREBF1 complex, which is reinforced by structural analysis. Thus, CRTC2/SREBF1 complex plausibly modulate the transcription of multiple proteins that fine-tune the cellular metabolism under metabolic stress.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:12:16.797.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039817
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Muhammad Ayaz Anwar
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-03 02:06:43	ID requested
1	2023-10-24 13:16:39	announced
2	2023-11-14 09:11:48	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:12:17	announced	2024-10-22: Updated project metadata.

10.1016/j.mcpro.2023.100637;

10.6019/PXD039817;

Lim JM, Anwar MA, Han HS, Koo SH, Kim KP, CREB-Regulated Transcriptional Coactivator 2 Proteome Landscape is Modulated by SREBF1. Mol Cell Proteomics, 22(10):100637(2023) [pubmed]

submitter keyword: CRTC2, PPI, metabolic disorders, SREBF1, glucose homeostasis

Kwang Pyo Kim
contact affiliation	Department of Applied Chemistry, Institute of Natural Science, Global Center for Pharmaceutical Ingredient Materials, Kyung Hee University, Yongin, 17104, South Korea.
contact email	kimkp@khu.ac.kr
lab head
Muhammad Ayaz Anwar
contact affiliation	Kyung Hee University, Global Campus
contact email	ayazanwar@khu.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039817
     1. Label: PRIDE project
     2. Name: CREB regulated transcriptional coactivator 2 proteome landscape is plausibly modulated by SREBF1