PXD039788

PXD039788 is an original dataset announced via ProteomeXchange.

Title	Comprehensive proteomic characterization identifies potential molecular targets for therapy and imaging in the Cluster 1 human pheochromocytoma and paraganglioma
Description	In order to identify new proteins with a potential to become targets for therapy or tumor imaging in pheochromocytoma and paraganglioma, we performed detailed proteomic analysis of 22 well-characterized human PPGL samples and normal chromaffin tissue from adrenal medulla. A standard quantitative proteomic analysis of tumor tissue was accompanied by specific membrane proteome-aimed methods, namely glycopeptide capture using lectin-affinity (Zielinska 2010) or hydrazide chemistry (Tian 2007) and enrichment of membrane-embedded hydrophobic transmembrane segment (Vit et al 2016 ). The parallel application of these complementary methods recently presented as “Pitchfork strategy”, enables deep quantitative profiling of human membrane proteomes without sacrificing the information on soluble non-membrane proteins (Vit et al 2019).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:10:37.634.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ondrej Vit
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	homoserine lactone; homoserine; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-01 05:34:36	ID requested
1	2023-09-25 08:44:42	announced
⏵ 2	2023-11-14 08:10:38	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: surface proteins, pheochromocytoma, paraganglioma, pitchfork strategy, membrane proteome,human

Jiří Petrák
contact affiliation	BIOCEV, First Faculty of Medicine, Charles University, Czech Republic
contact email	jpetr@lf1.cuni.cz
lab head
Ondrej Vit
contact affiliation	Charles University, First Faculty of Medicine, BIOCEV
contact email	ondrvit@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD039788

PRIDE project URI

• PRIDE
  1. PXD039788
     1. Label: PRIDE project
     2. Name: Comprehensive proteomic characterization identifies potential molecular targets for therapy and imaging in the Cluster 1 human pheochromocytoma and paraganglioma