PXD039786

PXD039786 is an original dataset announced via ProteomeXchange.

Title	Actb knockout in primary mouse embryonic fibroblasts LC-MS/MS
Description	The cytoplasmic actin proteins, beta- and gamma-actin, are 99% identical but perform non-redundant functions. Genes encoding the cytoplasmic actins, Actb and Actg1, respectively, are less similar but still share 89% of their nucleotide sequences. Knockout (KO) of Actb by deletion of first coding exons 2 and 3 in mice is embryonic lethal while KO embryonic fibroblasts (MEFs) fail to proliferate. In contrast, KO of Actg1 is viable but mice lacking Actg1 present with increased perinatal lethality and Actg1 KO MEFs present with a much milder defect in cell proliferation. Recent studies have identified important protein-independent functions for both Actb and Actg1 and demonstrate that deletions within the Actb nucleotide sequence, and not loss of the beta-actin protein, cause the most severe phenotypes in KO mice and cells. Here, we use a multi-omics approach to better understand what drives the phenotypes of Actb KO MEFs. RNA-sequencing and mass spectrometry of Actb KO MEFs reveal largescale changes to the transcriptome, proteome, and phosphoproteome. Pathway analysis of genes and proteins differentially expressed upon Actb KO shows widespread dysregulation of genes involved in the cell cycle. Together, these data suggest novel, protein-independent roles for Actb in regulating gene expression associated with control of cell proliferation.
HostingRepository	PRIDE
AnnounceDate	2024-02-19
AnnouncementXML	Submission_2024-02-19_15:38:39.240.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039786
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lauren Sundby
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-01 05:33:56	ID requested
⏵ 1	2024-02-19 15:38:39	announced

10.6019/PXD039786;

10.1016/J.EJCB.2024.151397;

submitter keyword: actb,actin, actin isoforms, beta actin

James M Ervasti
contact affiliation	Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota
contact email	jervasti@umn.edu
lab head
Lauren Sundby
contact affiliation	Unviersity of Minnesota
contact email	sundb217@umn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD039786

PRIDE project URI

• PRIDE
  1. PXD039786
     1. Label: PRIDE project
     2. Name: Actb knockout in primary mouse embryonic fibroblasts LC-MS/MS