PXD039766

PXD039766 is an original dataset announced via ProteomeXchange.

Title	The secretome features associated with the development of re-sistance to vemurafenib in BRAFV600E mutated colon cancer cells
Description	Patients with metastatic colorectal cancer (mCRC) carrying BRAFV600E mutation have worse response to chemotherapy and poor prognosis. The aim of this study was to conduct for the first time comparative proteomics profiling of the secretome from vemurafenib-sensitive vs. resistant colon cancer cells harboring BRAFV600E mutation in order to identify specific secretory features potentially associated with the development of resistance to vemurafenib. Towards this aim, we employed a label-free quantitative LC-MS/MS analysis. Obtained results pointed to aberrant regulation of DNA replication and the endoplasmic reticulum stress as the major secretome features associated with chemoresistant phenotype. Further studies are required to investigate diagnostic values of these proteins in the clinical setting and to examine their roles in acquired resistance to BRAF inhibition not only in colon cancer but also in other BRAF mutated cancers.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:45:16.435.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antje Dittmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-01 05:05:11	ID requested
1	2023-04-29 00:39:00	announced
⏵ 2	2023-11-14 08:45:22	announced	2023-11-14: Updated project metadata.

Car I, Dittmann A, Klobu, č, ar M, Grb, č, i, ć P, Kraljevi, ć, Paveli, ć S, Sedi, ć M, Secretome Screening of BRAFV600E-Mutated Colon Cancer Cells Resistant to Vemurafenib. Biology (Basel), 12(4):(2023) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: BRAFV600E, BRAF inhibition, HSPA5/ GRP78, colon cancer, RPA1, ER stress, vemurafenib, secretome, chemoresistance, DNA replication, LC-MS/MS

Mirela Sedic
contact affiliation	Centre for Applied Bioanthropology, Institute for Anthropological Research, Zagreb, Croatia
contact email	mirela.sedic@inantro.hr
lab head
Antje Dittmann
contact affiliation	ETH
contact email	antje.dittmann@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD039766

PRIDE project URI

• PRIDE
  1. PXD039766
     1. Label: PRIDE project
     2. Name: The secretome features associated with the development of re-sistance to vemurafenib in BRAFV600E mutated colon cancer cells