PXD039755

PXD039755 is an original dataset announced via ProteomeXchange.

Title	Data Independent Acquisition (DIA) based quantification workflow for proteome analysis of GASH/Sal and Control hamster
Description	The GASH/Sal hamster (Genetic audiogenic seizure, Salamanca) is a model of audiogenic seizures with the epileptogenic focus localized in the inferior colliculus (IC). The sound-induced seizures exhibit a short latency (7-9 seconds), which implies innate protein disturbances in the IC as a basis for seizure susceptibility and generation. Here, we aim to study the protein profile in the GASH/Sal IC in comparison to controls. Protein samples from the IC were processed for enzymatic digestion and then analyzed by mass spectrometry in Data-Independent Acquisition mode. After identifying the proteins using the UniProt database, we selected those with differential expression. We identified 5254 proteins, of which 184 were differentially expressed, 126 upregulated and 58 downregulated. Moreover, a small number of proteins were uniquely found in the GASH/Sal or the control. The resuls indicated a protein profile alteration in the epileptogenic nucleus that might underlie the innate occuring audiogenic seizures in the GASH/Sal model.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:43.511.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carlos García
SpeciesList	scientific name: Mesocricetus auratus (Golden hamster); NCBI TaxID: 10036;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Thermo Scientific instrument model; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-01 03:53:01	ID requested
1	2023-05-10 14:51:26	announced
⏵ 2	2023-11-14 08:47:53	announced	2023-11-14: Updated project metadata.

Garc, í, a-Peral C, Ledesma MM, Herrero-Turri, ó, n MJ, G, ó, mez-Nieto R, Castellano O, L, ó, pez DE, Proteomic and Bioinformatic Tools to Identify Potential Hub Proteins in the Audiogenic Seizure-Prone Hamster GASH/Sal. Diagnostics (Basel), 13(6):(2023) [pubmed]

submitter keyword: MaxQuant, MSstats,DIA, DDA, mProphet

Maria Dolores Estilita López García
contact affiliation	Department of Cellular Biology and Pathology, Instituto de Neurociencias de Castilla y León (INCyL), Laboratory Nº12
contact email	lopezde@usal.es
lab head
Carlos García
contact affiliation	USAL (INCYL), Salamanca
contact email	carlosp@usal.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039755
     1. Label: PRIDE project
     2. Name: Data Independent Acquisition (DIA) based quantification workflow for proteome analysis of GASH/Sal and Control hamster