PXD039725

PXD039725 is an original dataset announced via ProteomeXchange.

Title	A draft map of the human sputum proteome
Description	Chronic respiratory diseases are a growing burden, and potentially life-threatening condition if left untreated. It is an urgent medical concern that requires proper diagnosis, treatment, and management to prevent serious complications. Analyzing induced sputum is a non-invasive approach for investigating the mechanisms and progression of respiratory diseases. It allows for the collection of sputum samples without disrupting the patient's normal life. By analyzing the proteins and other molecular substances present in the sputum, it can provide valuable insights into the pathological changes and evaluate the effectiveness of treatments for respiratory diseases. In this study, we carried out proteomic analysis of three types of sputum collected from patients with chronic respiratory diseases using three different digestion methods. In comparison, FASP method was found to be more efficient than In-gel digestion and in-solution digestions, yielding the highest number of unique protein identifications. Integrate the proteomic and phosphoproteomic profiling of three types of sputum, it was observed that raw sputum samples exhibited a higher number of unique protein and phosphoprotein identifications. Our study provides valuable insights into the application of proteomics to the analysis of sputum samples, and serves as a useful resource for understanding the potential of this approach in the study of respiratory diseases.
HostingRepository	iProX
AnnounceDate	2023-01-28
AnnouncementXML	Submission_2023-01-27_19:18:17.134.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoyin Zeng
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-27 19:17:56	ID requested
⏵ 1	2023-01-27 19:18:17	announced

Dataset with its publication pending

submitter keyword: chronic respiratory diseases, sputum proteome, quantitative proteomics, digestion comparison, sample preparation techniques

Fei Long
contact affiliation	Guangzhou Medical University
contact email	long_fei78@hotmail.com
lab head
Xiaoyin Zeng
contact affiliation	Guangzhou Medical University
contact email	1280823006@qq.com
dataset submitter

iProX dataset URI