PXD039719

PXD039719 is an original dataset announced via ProteomeXchange.

Title	EED and IgG (as control) co-immunoprecipitation of wild type embryonic stem cells (ESCs), with 3 biological replicates per condition.
Description	DNA and Histone-3 Lysine 27 methylation typically function as repressive modifications and operate within distinct genomic compartments. In mammals, the majority of the genome is kept in a DNA methylated state, whereas the Polycomb Repressive Complexes regulate the unmethylated CpG-rich promoters of developmental genes. In contrast to this general framework, the extraembryonic lineages display non-canonical, globally intermediate DNA methylation levels that includes disruption of local Polycomb domains. To better understand this unusual landscape’s molecular properties, we genetically and chemically perturbed major epigenetic pathways in mouse Trophoblast Stem Cells (TSCs). We find that the extraembryonic epigenome reflects ongoing and dynamic de novo methyltransferase recruitment, which is continuously antagonized by Polycomb to maintain intermediate, locally disordered methylation. Despite its disorganized molecular appearance, our data point to a highly controlled equilibrium between counteracting repressors within extraembryonic cells, one that can seemingly persist indefinitely without bistable features typically seen for embryonic forms of epigenetic regulation.
HostingRepository	PRIDE
AnnounceDate	2023-02-06
AnnouncementXML	Submission_2023-02-06_01:35:16.826.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DavidMeierhofer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF SCP

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-27 10:53:47	ID requested
⏵ 1	2023-02-06 01:35:17	announced

Dataset with its publication pending

submitter keyword: epigenetic regulation,mouse embryonic stem cells, EED, Polycomb

AlexanderMeissner
contact affiliation	Department of Genome Regulation, Max Planck Institute for Molecular Genetics; Berlin, Germany
contact email	meissner@molgen.mpg.de
lab head
DavidMeierhofer
contact affiliation	Mass Spectrometry Facility MPIMG
contact email	meierhof@molgen.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD039719

PRIDE project URI

• PRIDE
  1. PXD039719
     1. Label: PRIDE project
     2. Name: EED and IgG (as control) co-immunoprecipitation of wild type embryonic stem cells (ESCs), with 3 biological replicates per condition.