PXD039714

PXD039714 is an original dataset announced via ProteomeXchange.

Title	Enzyme adaptation to habitat thermal legacy shapes the thermal plasticity of marine microbiomes
Description	Microbial communities respond to temperature with physiological adaptation and compositional turnover. Whether thermal selection of enzymes explains marine microbiome plasticity in response to temperature remains unresolved. By quantifying the thermal behaviour of seven functionally-independent enzyme classes (esterase, extradiol dioxygenase, phosphatase, beta-galactosidase, nuclease, transaminase, and aldo-keto reductase) in native proteomes of marine sediment microbiomes from the Irish Sea to the southern Red Sea, we record a significant effect of the mean annual temperature (MAT) on enzyme’s response (R2, 0.51–0.80, p < 0.01 in all cases). Activity and stability profiles of 228 esterases and 5 extradiol dioxygenases from sediment and seawater across 70 locations worldwide (latitude 62.2°S–16°N, MAT –1.4ºC–29.5ºC) validate this thermal pattern. Modelling the esterase phase transition temperature as a measure of structural flexibility, confirm the observed relationship with MAT. Furthermore, when considering temperature variability in sites with non-significantly different MATs, the broadest range of enzyme thermal behaviour and the highest growth plasticity of the enriched heterotrophic bacteria occur in samples with the widest annual thermal variability. These results indicate that temperature-driven enzyme selection shapes microbiome thermal plasticity and that thermal variability finely tunes such processes and should be considered alongside MAT in forecasting microbial community thermal response
HostingRepository	PRIDE
AnnounceDate	2023-02-07
AnnouncementXML	Submission_2023-02-07_10:26:37.740.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039714
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	SergioCiordia
SpeciesList	scientific name: Eubacteriales; NCBI TaxID: 186802;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-27 10:52:35	ID requested
⏵ 1	2023-02-07 10:26:38	announced

10.6019/PXD039714;

submitter keyword: Microbial physiology, Climate changes, Enzymatic activity, Temperature variability, Thermal response,Marine bacteria

DanieleDaffonchio
contact affiliation	Biological and Environmental Sciences and Engineering Division (BESE), Red Sea Research Centre (RSRC), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia
contact email	Daniele.Daffonchio@kaust.edu.sa
lab head
SergioCiordia
contact affiliation	Spanish National Center for Biotechnology
contact email	sciordia@cnb.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD039714

PRIDE project URI

• PRIDE
  1. PXD039714
     1. Label: PRIDE project
     2. Name: Enzyme adaptation to habitat thermal legacy shapes the thermal plasticity of marine microbiomes