PXD039706 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | BRWD3 targets KDM5/Lid for degradation to maintain H3K4 methylation levels |
Description | Histone modifications are critical for regulating chromatin structure and gene expression. Dysregulation of histone modification levels may contribute to disease development and cancer. Therefore, understanding histone modifications is essential for development and disease. The chromatin-binding protein BRWD3, a known substrate-specificity factor of the Cul4-DDB1 E3 ubiquitin ligase complex, is required for maintaining active histone modification levels. Loss of BRWD3 function causes an increase in H3K4me1 levels. The underline mechanism, however, is unknown. We found that BRWD3 depletion also causes a decrease in H3K4me3 levels. To reveal the mechanism by which BRWD3 regulates the H3K4 methylation levels, we performed BRWD3-IP mass-spectrometry. We identified an interaction between BRWD3 and the lysine-specific demethylase 5 (KDM5/Lid), an enzyme that removes tri- and di- methyl marks from lysine 4 on histone H3. Moreover, analysis of ChIP-seq data revealed that BRWD3 and KDM5 are significantly co-localized throughout the genome. We show that BRWD3 promotes K48-linked ubiquitination of KDM5. Consistent with this, KDM5/Lid is rapidly degraded in a proteasome-dependent manner with a half-life of less than 30 mins. Critically, KDM5/Lid degradation is dependent on both BRWD3 and Cul4. In addition, we have found that BRWD3 is suppressor of Position-effect variegation (PEV). Loss of a single copy of KDM5, however, partially rescues the the BRWD3 PEV phenotype. Our results suggest that BRWD3 targets KDM5/Lid for degradation to ensure the balance of H3K4me levels. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:05:21.258.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jonathan Davies |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-27 10:50:22 | ID requested | |
1 | 2023-09-19 15:19:12 | announced | |
⏵ 2 | 2023-11-14 09:05:24 | announced | 2023-11-14: Updated project metadata. |
Publication List
Han D, Schaffner SH, Davies JP, Benton ML, Plate L, Nordman JT, BRWD3 promotes KDM5 degradation to maintain H3K4 methylation levels. Proc Natl Acad Sci U S A, 120(39):e2305092120(2023) [pubmed] |
Keyword List
submitter keyword: H3K4, histone, degradation, E3 ubiquitin ligase,BRWD3 |
Contact List
Jared Nordman |
contact affiliation | Department of Biological Sciences, Vanderbilt University |
contact email | jared.nordman@vanderbilt.edu |
lab head | |
Jonathan Davies |
contact affiliation | Vanderbilt University |
contact email | jonathan.p.davies@vanderbilt.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039706
- Label: PRIDE project
- Name: BRWD3 targets KDM5/Lid for degradation to maintain H3K4 methylation levels