PXD039679
PXD039679 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analyses identified distinct types of vesicles essential for egress of Plasmodium falciparum gametocytes from red blood cells |
| Description | Transmission of malaria parasites to the mosquito is mediated by sexual precursor cells, the gametocytes. Upon entering the mosquito midgut, the gametocytes egress from the enveloping erythrocyte while passing through gametogenesis. Egress follows an inside-out mode during which the membrane of the parasitophorous vacuole ruptures prior to the erythrocyte membrane. Membrane rupture requires the exocytosis of specialized secretory vesicles of the parasites; i.e. the osmiophilic bodies (OBs) involved in rupture of the parasitophorous vacuole membrane, and vesicles (here termed g-exonemes) that harbour the perforin-like protein PPLP2 needed for erythrocyte lysis. While several OB proteins are known, like G377 and MDV1/Peg3, the protein composition of the g-exonemes remains unclear. Here, we used high-resolution imaging and BioID methods to study the two types of egress vesicles in Plasmodium falciparum gametocytes. We show that OB exocytosis precedes discharge of the g-exonemes and that exocytosis of the g-exonemes, but not of the OBs, is calcium-sensitive. Further, the two types of vesicles exhibit distinct proteomes. In addition to known egress-related proteins, our analyses revealed novel markers of OBs and g-exonemes, including proteins involved in vesicle trafficking. Our data provide insight into the immense molecular machinery required for the inside-out egress of P. falciparum gametocytes. |
| HostingRepository | jPOST |
| AnnounceDate | 2024-01-25 |
| AnnouncementXML | Submission_2024-01-24_07:10:26.346.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Malte Sielaff |
| SpeciesList | scientific name: Plasmodium falciparum; NCBI TaxID: 5833; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-01-26 00:33:58 | ID requested | |
| 1 | 2024-01-24 07:00:19 | announced | |
| ⏵ 2 | 2024-01-24 07:10:27 | announced | 2024-01-25: Updated PubMed. |
Publication List
| Sassmannshausen J, Bennink S, Distler U, K, ΓΌ, chenhoff J, Minns AM, Lindner SE, Burda PC, Tenzer S, Gilberger TW, Pradel G, Comparative proteomics of vesicles essential for the egress of Plasmodium falciparum gametocytes from red blood cells. Mol Microbiol, 121(3):431-452(2024) [pubmed] |
Keyword List
| submitter keyword: Plasmodium falciparum, malaria, gametocyte, egress, red blood cell, vesicle |
Contact List
| Stefan Tenzer | |
|---|---|
| lab head | |
| Malte Sielaff | |
| contact affiliation | University Medical Center of the Johannes Gutenberg-University Mainz |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002011/ |
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