PXD039669

PXD039669 is an original dataset announced via ProteomeXchange.

Title	TREx-BCBL1-RTA GPRC5A-FLAG Immunoprecipitation LC-MS
Description	The epitranscriptomic modification m6A is a ubiquitous feature of the mammalian transcriptome. It modulates mRNA fate and dynamics to exert regulatory control over numerous cellular processes and disease pathways, including viral infection. Kaposi’s sarcoma-associated herpesvirus (KSHV) reactivation from the latent phase leads to redistribution of m6A topology upon both viral and cellular mRNAs within infected cells. Here we investigate the role of m6A in cellular transcripts upregulated during KSHV lytic replication. Results show that m6A is crucial for the stability of the GPRC5A mRNA, whose expression is induced by the KSHV latent-lytic switch master regulator, the replication and transcription activator (RTA) protein. Moreover, we demonstrate that GPRC5A is essential for efficient KSHV lytic replication by directly regulating NFκB signalling. Overall, this work highlights the central importance of m6A in modulating cellular gene expression to influence viral infection.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:03:15.681.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Timothy Mottram
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human gammaherpesvirus 8; NCBI TaxID: NCBITaxon:37296;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-25 09:08:52	ID requested
1	2023-07-20 11:19:08	announced
⏵ 2	2023-11-14 09:03:17	announced	2023-11-14: Updated project metadata.

Manners O, Baquero-Perez B, Mottram TJ, Yonchev ID, Trevelyan CJ, Harper KL, Menezes S, Patterson MR, Macdonald A, Wilson SA, Aspden JL, Whitehouse A, A Regulates the Stability of Cellular Transcripts Required for Efficient KSHV Lytic Replication. Viruses, 15(6):(2023) [pubmed]

submitter keyword: human KSHV GPRC5A

Adrian Whitehouse
contact affiliation	School of Molecular and Cellular Biology, Faculty of Biological Sciences and Astbury Centre of Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom
contact email	A.Whitehouse@leeds.ac.uk
lab head
Timothy Mottram
contact affiliation	University of Leeds
contact email	t.mottram@leeds.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD039669

PRIDE project URI

• PRIDE
  1. PXD039669
     1. Label: PRIDE project
     2. Name: TREx-BCBL1-RTA GPRC5A-FLAG Immunoprecipitation LC-MS