PXD039665

PXD039665 is an original dataset announced via ProteomeXchange.

Title	The proteome of hand eczema assessed by tape stripping
Description	Hand eczema (HE) is a prevalent skin disease. However, classification of HE into different subtypes remains challenging. Limited number of prior studies have employed invasive biopsy-based strategies; yet, studies of the HE proteome using non-invasive tape stripping methodology have not been reported. In this study, we wanted to assess whether global proteomic analysis of skin tape strip samples can be used for sub-classification of HE patients. Tape strips were collected from patients with HE and healthy skin. Liquid chromatography–mass spectrometry (LC/MS) proteomics was performed, and the global protein expression was analyzed. We identified 2,919 proteins in stratum corneum-derived skin cells from tape strip samples. Compared to healthy skin, the lesional samples from HE patients exhibited increased expression of immune-related markers and a decreased expression of structural barrier proteins. The difference between HE subtypes was restricted to the lesional skin areas, and included an increased expression of skin barrier-related proteins independently of the concurrent AD. In conclusion we found, that the non-invasive tape strip method used in combination with LC/MS proteomics can be used for analysis of skin protein expression in HE patients. Thus, the method shows potential for assessing the proteomic differences between subtypes of HE, and biomarker discovery.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:45:04.304.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julie Breinholt Kjær Sølberg
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-25 08:25:09	ID requested
1	2023-04-26 16:36:50	announced
⏵ 2	2023-11-14 08:45:07	announced	2023-11-14: Updated project metadata.

S, ø, lberg JBK, Quaade AS, Drici L, Sulek K, Ulrich NH, L, ø, vendorf MB, Thyssen JP, Mann M, Dyring-Andersen B, Johansen JD, The Proteome of Hand Eczema Assessed by Tape Stripping. J Invest Dermatol, 143(8):1559-1568.e5(2023) [pubmed]

submitter keyword: proteome, subtypes, atopic dermatitis,Hand eczema, Tape stripping

Beatrice Dyring-Andersen
contact affiliation	Novo Nordisk Foundation (NNF) Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark, and Department of Dermatology and Allergy Herlev-Gentofte Hospital, University of Copenhagen Hellerup, Denmark.
contact email	beatrice.dyring-andersen@regionh.dk
lab head
Julie Breinholt Kjær Sølberg
contact affiliation	Department of allergy and skin, Gentofte Hospital, Gentofte
contact email	julie.soelberg@regionh.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD039665

PRIDE project URI

• PRIDE
  1. PXD039665
     1. Label: PRIDE project
     2. Name: The proteome of hand eczema assessed by tape stripping