PXD039661

PXD039661 is an original dataset announced via ProteomeXchange.

Title	Isolation, enrichment and identification of p62 condensates components by GFP-Trap Agarose Beads pulldown followed by Label Free and Tandem Mass Tag (TMT) mass spectrometry analysis from Hap1 cells
Description	Selective autophagy contributes to the removal of harmful proteins from the cytoplasm. This cargo material is selected by cargo receptors such as p62/SQSTM1 and finally degraded. However, the molecular composition of the p62 condensates and therefore the nature of the cargo delivered by them is incompletely understood. In order to obtain insights into their composition, we have developed a method to isolate these condensates followed by TMT and Label Free mass spectrometry-based identification of their protein content.
HostingRepository	PRIDE
AnnounceDate	2023-11-22
AnnouncementXML	Submission_2023-11-22_05:53:43.350.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	WeiQiang Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-25 08:06:07	ID requested
⏵ 1	2023-11-22 05:53:43	announced

Dataset with its publication pending

submitter keyword: Autophagy

cargo receptor

SQSTM1

translation

Sascha Martens
contact affiliation	Max Perutz Labs, University of Vienna, Vienna BioCenter, Dr Bohr-Gasse 9/3,
contact email	sascha.martens@univie.ac.at
lab head
WeiQiang Chen
contact affiliation	Mass Spectrometry Facility, Max Perutz Laboratories Support GmbH
contact email	chenweiqiang@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD039661

PRIDE project URI

• PRIDE
  1. PXD039661
     1. Label: PRIDE project
     2. Name: Isolation, enrichment and identification of p62 condensates components by GFP-Trap Agarose Beads pulldown followed by Label Free and Tandem Mass Tag (TMT) mass spectrometry analysis from Hap1 cells