PXD039658

PXD039658 is an original dataset announced via ProteomeXchange.

Title	Acetylation Coordinates the Crosstalk between Carbon Metabolism and Ammonium Assimilation
Description	Enteric bacteria use up to 15% of their cellular energy for ammonium assimilation via glutamine synthetase (GS)/glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH), in response to low or high ammonium availability. However, the sensory mechanisms for effective and appropriate coordination between carbon metabolism and ammonium assimilation are not fully elucidated. Here, we report that, in Salmonella, carbon metabolism coordinates the activities of GS/GDH via functional reversible protein lysine acetylation. Glucose simultaneously promotes acetyltransferase Pat-mediated acetylation on Lys164 and Lys353 of GS to activate the adenylylated-GS by inducing its conformation change, while on Lys128 of GDH to inactivate the enzyme by impeding its catalytic center, respectively, which are reversed by deacetylase CobB-mediated deacetylation. Molecular dynamic (MD) simulations indicated that the acetylation activation of GS activity was adenylylation-dependent. Acetylation and deacetylation occur within minutes of ‘glucose shock’ to promptly adapt to ammonium/carbon variation and finely balance glutamine/glutamate synthesis. Acetylation can rehabilitate the growth tardiness of Salmonella mutant with chromosomal mimetic mutation of adenylylated-GS and thus help its survival in mice. Thus, glucose-driven acetylation integrates the signals of assimilation and carbon metabolism for proper growth control.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:23.184.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qijun Wang
SpeciesList	scientific name: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720); NCBI TaxID: 99287;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-25 06:25:33	ID requested
1	2023-06-20 17:45:59	announced
⏵ 2	2023-11-14 09:01:26	announced	2023-11-14: Updated project metadata.

Sun Y, Zhang Y, Zhao T, Luan Y, Wang Y, Yang C, Shen B, Huang X, Li G, Zhao S, Zhao GP, Wang Q, Acetylation coordinates the crosstalk between carbon metabolism and ammonium assimilation in Salmonella enterica. EMBO J, 42(13):e112333(2023) [pubmed]

submitter keyword: Ammonium assimilation,Acetylation, Coordination, Carbon metabolism

Qijun Wang
contact affiliation	Shanghai Jiao Tong University School of Medicine
contact email	qijun_wang@sjtu.edu.cn
lab head
Qijun Wang
contact affiliation	Shanghai Jiao Tong University
contact email	qijun_wang@sjtu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039658
     1. Label: PRIDE project
     2. Name: Acetylation Coordinates the Crosstalk between Carbon Metabolism and Ammonium Assimilation