PXD039653

PXD039653 is an original dataset announced via ProteomeXchange.

Title	Comprehensive proteome analysis of propolis elucidates the origin and function of its proteins
Description	Propolis is a resinous honeybee product, rich in polyphenolic compounds and with high economic value. Although extensive studies regarding the chemical composition of different propolis extracts have been carried out, the propolis proteome remained unknown. The present study aimed at characterizing the proteome of two geographically-distinct propolis originating from Belgium and Iran. Propolis extracts were analysed by SDS-PAGE followed by MALDI-TOFand LC/ESI- mass spectrometry (MS). Our in-depth proteomic analysis led to the identification of honeybee venom and royal jelly proteins, and numerous plant-defense proteins in propolis, extending our understanding on the antimicrobial properties of this complex natural substance. The proteome of both propolis was mainly made up of proteins belonging to the poplar tree. The poplar PR-2 (β-1,3-glucanases) and PR-8 (acidic endochitinases) proteins were found to be the major components of both propolis extracts, while bee venom (Api m 1) and royal jelly allergens (MJRP 1and 2) were only identified in the propolis from Belgium. Overall, our proteome analyses revealed that propolis extracts from Belgium and Iran shared a core protein composition originating from the poplar proteome. The majority of identified proteins are involved in the plant defense against pathogens, some belonging to well-known pathogenesis-related families.
HostingRepository	PRIDE
AnnounceDate	2026-03-26
AnnouncementXML	Submission_2026-03-26_06:57:22.940.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastien Planchon
SpeciesList	scientific name: Apis mellifera (Honeybee); NCBI TaxID: NEWT:7460; scientific name: Viridiplantae; NCBI TaxID: NEWT:33090;
ModificationList	L-kynurenine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	5800 TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-25 06:14:43	ID requested
⏵ 1	2026-03-26 06:57:23	announced

10.1016/J.JFCA.2023.105869;

submitter keyword: honeybee

poplar,Bee product, phospholipase

bee venom, propolis

proteomics

Youcef Shahali
contact affiliation	CHU de Besancon, Besancon, France
contact email	yshahali@chu-besancon.fr
lab head
Sebastien Planchon
contact affiliation	Luxembourg Institute of Science and Technology
contact email	sebastien.planchon@list.lu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039653
     1. Label: PRIDE project
     2. Name: Comprehensive proteome analysis of propolis elucidates the origin and function of its proteins