PXD039648

PXD039648 is an original dataset announced via ProteomeXchange.

Title	A positive feedback loop controls Toxoplasma chronic differentiation
Description	Successful infection strategies must balance pathogen amplification and persistence. In Toxoplasma gondii, this is accomplished through differentiation into dedicated cyst-forming chronic stages that avoid clearance by the host immune system. The transcription factor BFD1 is both necessary and sufficient for stage conversion; however, its regulation is not understood. We examine five factors transcriptionally activated by BFD1. One of these is a cytosolic RNA-binding protein of the CCCH-type zinc finger family, which we name BFD2. Parasites lacking BFD2 fail to induce BFD1 and are consequently unable to fully differentiate in culture or in mice. BFD2 interacts with the BFD1 transcript under stress and deletion of BFD2 reduces BFD1 protein levels, but not mRNA abundance. The reciprocal effects on BFD2 transcription and BFD1 translation outline a positive feedback loop that enforces commitment to differentiation. BFD2 helps explain how parasites commit to the chronic gene-expression program and elucidates how the balance between proliferation and persistence is achieved over the course of infection.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:04:30.127.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039648
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alex Chan
SpeciesList	scientific name: Toxoplasma gondii ME49; NCBI TaxID: 508771;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-25 03:35:01	ID requested
1	2023-08-07 11:06:43	announced
⏵ 2	2023-11-14 09:04:38	announced	2023-11-14: Updated project metadata.

10.6019/PXD039648;

Licon MH, Giuliano CJ, Chan AW, Chakladar S, Eberhard JN, Shallberg LA, Chandrasekaran S, Waldman BS, Koshy AA, Hunter CA, Lourido S, A positive feedback loop controls Toxoplasma chronic differentiation. Nat Microbiol, 8(5):889-904(2023) [pubmed]

submitter keyword: T. gondii, LC-MSMS, parasite development, gene expression

Sebastian Lourido
contact affiliation	Associate Professor, Department of Biology, Massachusetts Institute of Technology Whitehead Institute of Biomedical Research
contact email	lourido@wi.mit.edu
lab head
Alex Chan
contact affiliation	Whitehead Institute for Biomedical Research Department of Biology, Massachusetts Insitute of Technology
contact email	awchan@wi.mit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD039648

PRIDE project URI

• PRIDE
  1. PXD039648
     1. Label: PRIDE project
     2. Name: A positive feedback loop controls Toxoplasma chronic differentiation