PXD039627

PXD039627 is an original dataset announced via ProteomeXchange.

Title	ChIP-MS reveals the local chromatin composition by label-free quantitative proteomics
Description	Chromatin immunoprecipitation (ChIP) has been a cornerstone for epigenetic analyses over the last decades, but even coupled to sequencing approaches (ChIP-seq), it is ultimately limited to one protein at a time. In a complementary effort, we here combined ChIP with label-free quantitative (LFQ) mass spectrometry (ChIP-MS) to interrogate local chromatin compositions. We demonstrate the versality of our approach at telomeres, with transcription factors, in tissue and by dCas9-driven locus-specific enrichment.
HostingRepository	PRIDE
AnnounceDate	2024-03-05
AnnouncementXML	Submission_2024-03-05_01:27:41.719.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dennis Kappei
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; timsTOF fleX

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-24 08:19:24	ID requested
⏵ 1	2024-03-05 01:27:42	announced

10.1101/2023.01.27.525999;

submitter keyword: ChIP-MS, dCas9, telomeres, LFQ, MYB

Dennis Kappei
contact affiliation	Cancer Science Institute of Singapore, National University of Singapore
contact email	dennis.kappei@nus.edu.sg
lab head
Dennis Kappei
contact affiliation	Cancer Science Institute of Singapore
contact email	dennis.kappei@nus.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD039627

PRIDE project URI

• PRIDE
  1. PXD039627
     1. Label: PRIDE project
     2. Name: ChIP-MS reveals the local chromatin composition by label-free quantitative proteomics