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PXD039625

PXD039625 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSAPS3 is an AMPK inhibitor and controls metabolic homeostasis upon dietary challenge
DescriptionInhibition of AMPK is tightly associated with metabolic perturbations upon over nutrition, yet the molecular mechanism underneath that is not clear. Here, we demonstrate serine/threonine-protein phosphatase 6 regulatory subunit 3, SAPS3, is a negative regulator of AMPK. SAPS3 is induced under high fat diet (HFD) and recruits the PP6 catalytic subunit to deactivate phosphorylated-AMPK, thereby inhibiting AMPK-controlled metabolic pathways. Either whole-body or liver-specific deletion of SAPS3 protects male mice against the HFD-induced detrimental consequences and reverses HFD-induced metabolic and transcriptional alterations while loss of SAPS3 has no effects on mice under balanced diets. Furthermore, genetic inhibition of AMPK is sufficient to block the protective phenotype in SAPS3 knockout male mice under HFD. Together, our results reveal that SAPS3 is a negative regulator of AMPK and suppression of SAPS3 functions as a guardian when metabolism is perturbed and represents a potential therapeutic strategy to treat metabolic syndromes.
HostingRepositoryPRIDE
AnnounceDate2023-01-26
AnnouncementXMLSubmission_2023-01-26_01:29:50.260.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD039625
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterYingYang
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-24 08:18:13ID requested
12023-01-26 01:29:50announced
Publication List
10.6019/PXD039625;
Keyword List
submitter keyword: Human
Contact List
MeiKong
contact affiliationDepartment of Molecular Biology and Biochemistry,School of Biological Sciences, University of California, Irvine, United States
contact emailmeik1@uci.edu
lab head
YingYang
contact affiliationUniversity of California, Irvine
contact emailyingy33@uci.edu
dataset submitter
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Dataset FTP location
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