PXD039625 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SAPS3 is an AMPK inhibitor and controls metabolic homeostasis upon dietary challenge |
Description | Inhibition of AMPK is tightly associated with metabolic perturbations upon over nutrition, yet the molecular mechanism underneath that is not clear. Here, we demonstrate serine/threonine-protein phosphatase 6 regulatory subunit 3, SAPS3, is a negative regulator of AMPK. SAPS3 is induced under high fat diet (HFD) and recruits the PP6 catalytic subunit to deactivate phosphorylated-AMPK, thereby inhibiting AMPK-controlled metabolic pathways. Either whole-body or liver-specific deletion of SAPS3 protects male mice against the HFD-induced detrimental consequences and reverses HFD-induced metabolic and transcriptional alterations while loss of SAPS3 has no effects on mice under balanced diets. Furthermore, genetic inhibition of AMPK is sufficient to block the protective phenotype in SAPS3 knockout male mice under HFD. Together, our results reveal that SAPS3 is a negative regulator of AMPK and suppression of SAPS3 functions as a guardian when metabolism is perturbed and represents a potential therapeutic strategy to treat metabolic syndromes. |
HostingRepository | PRIDE |
AnnounceDate | 2023-01-26 |
AnnouncementXML | Submission_2023-01-26_01:29:50.260.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD039625 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | YingYang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-24 08:18:13 | ID requested | |
⏵ 1 | 2023-01-26 01:29:50 | announced | |
Publication List
Keyword List
Contact List
MeiKong |
contact affiliation | Department of Molecular Biology and Biochemistry,School of Biological Sciences, University of California, Irvine, United States |
contact email | meik1@uci.edu |
lab head | |
YingYang |
contact affiliation | University of California, Irvine |
contact email | yingy33@uci.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039625
- Label: PRIDE project
- Name: SAPS3 is an AMPK inhibitor and controls metabolic homeostasis upon dietary challenge