PXD039615

PXD039615 is an original dataset announced via ProteomeXchange.

Title	Multi-omics analysis of Bordetella pertussis fermentation
Description	Bordetella pertussis is the bacterial causative agent of whooping cough, a serious respiratory illness. An extensive knowledge on its virulence regulation and metabolism is a key factor to ensure pertussis vaccine manufacturing process robustness. The aim of this study was to refine our comprehension of B. pertussis physiology along the fermentation process. A longitudinal multi-omics analysis was carried out over a 26-hour small-scale fermentation of B. pertussis. Fermentations were performed in batch mode and under culture conditions intending to mimic industrial processes. Putative cysteine and proline starvations were respectively observed at the beginning of the exponential phase (from 4h to 8h) and during the exponential phase (18h45). As revealed by multi-omics analyses, the proline starvation induced major molecular changes, including a transient metabolism with internal stock consumption. In the meantime, growth and specific total PT, PRN and Fim2 antigen productions were negatively affected. Interestingly, the master virulence-regulating two-component system of B. pertussis (BvgASR) was not evidenced as the sole virulence regulator in this in vitro growth condition. Indeed, novel intermediate regulators were identified as putatively involved in the expression of some virulence-activated genes (vags). Such longitudinal multi-omics analysis applied to B. pertussis fermentation process emerges as a powerful tool for characterization and incremental optimization of vaccine antigen production.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:12.165.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039615
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paul Anziani
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251); NCBI TaxID: 257313;
ModificationList	reduced residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-23 07:43:37	ID requested
1	2023-03-11 12:00:21	announced
⏵ 2	2023-11-14 08:44:13	announced	2023-11-14: Updated project metadata.

10.6019/PXD039615;

Anziani P, Becker J, Mignon C, Arnaud-Barbe N, Courtois V, Izac M, Pizzato R, Abi-Ghanem J, Tran VD, Sarafian M, Bunescu A, Garnier D, Abachin E, Renauld-Mong, é, nie G, Guyard C, cultivated in bioreactors highlights medium starvations and transitory metabolisms, associated to vaccine antigen biosynthesis variations and global virulence regulation. Front Microbiol, 14():1036386(2023) [pubmed]

submitter keyword: Longitudinal multi-omics, Virulence, Fermentation molecular profiling, Antigen production, Proline starvation,Bordetella pertussis, Bacterial fermentation, Cysteine starvation

Cyril GUYARD
contact affiliation	CSO, Bioaster, France
contact email	cyril.guyard@bioaster.org
lab head
Paul Anziani
contact affiliation	Bioaster
contact email	paul.anziani@bioaster.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039615
     1. Label: PRIDE project
     2. Name: Multi-omics analysis of Bordetella pertussis fermentation