PXD039611

PXD039611 is an original dataset announced via ProteomeXchange.

Title	Dynamic antagonism between key repressive pathways maintains the placental epigenome -1
Description	DNA and Histone-3 Lysine 27 methylation typically function as repressive modifications and operate within distinct genomic compartments. In mammals, the majority of the genome is kept in a DNA methylated state, whereas the Polycomb Repressive Complexes regulate the unmethylated CpG-rich promoters of developmental genes. In contrast to this general framework, the extraembryonic lineages display non-canonical, globally intermediate DNA methylation levels that includes disruption of local Polycomb domains. To better understand this unusual landscape’s molecular properties, we genetically and chemically perturbed major epigenetic pathways in mouse Trophoblast Stem Cells (TSCs). We find that the extraembryonic epigenome reflects ongoing and dynamic de novo methyltransferase recruitment, which is continuously antagonized by Polycomb to maintain intermediate, locally disordered methylation. Despite its disorganized molecular appearance, our data point to a highly controlled equilibrium between counteracting repressors within extraembryonic cells, one that can seemingly persist indefinitely without bistable features typically seen for embryonic forms of epigenetic regulation. Dataset 1: EED co-immunoprecipitation of wild type mouse trophoblast stem cells (TSCs) and Eed knockout TSCs as control, with 3 biological replicates per condition.
HostingRepository	PRIDE
AnnounceDate	2023-01-27
AnnouncementXML	Submission_2023-01-27_01:26:59.640.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039611
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	DavidMeierhofer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-23 06:47:17	ID requested
⏵ 1	2023-01-27 01:27:00	announced

10.6019/PXD039611;

submitter keyword: mouse trophoblast stem cells, epigenetic regulation, EED, Polycomb

AlexanderMeissner
contact affiliation	Max Planck Institute for Molecular Genetics Department of Genome Regulation Ihnestraße 63-73 14195 Berlin, Germany
contact email	meissner@molgen.mpg.de
lab head
DavidMeierhofer
contact affiliation	Mass Spectrometry Facility MPIMG
contact email	meierhof@molgen.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039611
     1. Label: PRIDE project
     2. Name: Dynamic antagonism between key repressive pathways maintains the placental epigenome -1