PXD039610

PXD039610 is an original dataset announced via ProteomeXchange.

Title	Characterization and proteomic analysis of plasma EVs recovered from healthy and diseased dogs with Canine Leishmaniosis
Description	Dogs are highly valued companions and work animals that are susceptible to many life-threatening conditions like canine leishmaniosis (CanL). Plasma-derived extracellular vesicles (EVs), exploited extensively in biomarker discovery, constitute a mostly untapped resource in veterinary sciences. Thus, the definition of proteins associated with plasma EVs recovered from healthy and diseased dogs with a relevant pathogen would be important for biomarker development. For this, we recovered using size-exclusion chromatography (SEC), EVs from 19 healthy and 20 CanL dogs’ plasma and performed proteomic analysis by LC-MS/MS to define their core proteomic composition and search for CanL-associated alterations. EVs-specific markers were identified in all preparations and also non-EVs proteins. Some EVs markers like CD82 were specific to the healthy animals, while others like the Integrin beta 3 were identified in most samples. The EVs-enriched preparations allowed the identification of 529 canine proteins that were identified in both groups, while 465 and 154 were only identified in healthy or CanL samples, respectively. A GO enrichment analysis revealed few CanL-specific terms. Leishmania spp. protein identifications were also found alt-hough with only 1 unique peptide. Ultimately, CanL-associated proteins of interest were identified and a core proteome was revealed that will be available for intra- and inter-species comparisons.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:59.772.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nuno Santarém
SpeciesList	scientific name: Leishmania infantum; NCBI TaxID: 5671; scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-23 06:46:43	ID requested
1	2023-05-10 17:06:24	announced
⏵ 2	2023-11-14 08:48:02	announced	2023-11-14: Updated project metadata.

Esteves S, Lima C, Costa I, Os, ó, rio H, Fernandez-Becerra C, Santar, é, m N, Cordeiro-da-Silva A, Characterization and Proteomic Analysis of Plasma EVs Recovered from Healthy and Diseased Dogs with Canine Leishmaniosis. Int J Mol Sci, 24(6):(2023) [pubmed]

submitter keyword: Canine leishmaniosis

Leishmania infantum

extracellular vesicles

proteomics

Exosomes

Leishmaniasis

Leishmania

dogs

parasites

Anabela Cordeiro da Silva
contact affiliation	i3S - Institute for Research and Innovation in Health
contact email	cordeiro@ibmc.up.pt
lab head
Nuno Santarém
contact affiliation	i3S - Institute for Research and Innovation in Health
contact email	santarem@ibmc.up.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD039610

PRIDE project URI

• PRIDE
  1. PXD039610
     1. Label: PRIDE project
     2. Name: Characterization and proteomic analysis of plasma EVs recovered from healthy and diseased dogs with Canine Leishmaniosis