PXD039602
PXD039602 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Post translational modifications in spider major ampullate silk influence structural conformation and N-terminal dimerization dynamics |
| Description | Our current understanding of spider silk’s structure-function is largely based upon conclusions drawn from the comparison of amino acid sequences generated by genomics to techniques like NMR and X-ray crystallography. However, this comparison is ultimately limited to the extent that any given silk genome closely matches its proteome; with post-translational modifications (PTM) significantly influencing the structure and function of many proteins. This study utilizes phosphopeptide enrichment and mass spectrometry to produce a comprehensive PTM map across the major MaSp proteins of Trichonephila clavata, with PTMs observed in all structural domains. Solid-state NMR further confirms the presence and conformation of hydroxyproline, phosphoserine, phosphotyrosine and dityrosine, revealing phosphoserine’s roll in β-sheet inhibition. Moreover, N-terminal domain (NTD) phosphomimics where utilized to examine the impact of phosphorylation at the dimer interface, indicating that changes in charged clusters of the NTD monomers alters dimerization behavior in a manner that can possibly facilitate heterodimer formation. |
| HostingRepository | jPOST |
| AnnounceDate | 2024-01-23 |
| AnnouncementXML | Submission_2024-01-22_07:00:11.022.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Masaru Mori |
| SpeciesList | scientific name: cellular organisms; NCBI TaxID: 131567; |
| ModificationList | S-carboxamidomethyl-L-cysteine; Acetyl; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; monohydroxylated proline |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-01-22 18:32:21 | ID requested | |
| ⏵ 1 | 2024-01-22 07:00:11 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Trichonephila clavata, ampullate silk, spider silk |
Contact List
| Kazuharu Arakawa | |
|---|---|
| lab head | |
| Masaru Mori | |
| contact affiliation | Institute for Advanced Biosciences, Keio University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001986/ |
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