⮝ Full datasets listing

PXD039588

PXD039588 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome profiling highlights mechanisms underlying pigment and tocopherol accumulation in red and black rice seeds
DescriptionRice is a major component of the human diet and feeds more than 50 million people across the globe. Therefore, efforts are being made to improve the nutritional quality of rice seeds in order to make a super-rice cultivar rich in antioxidants and vitamins. We previously developed two rice cultivars, Super-hongmi (red seeds) and Super-jami (black seeds), that were highly rich in antioxidants and exhibited high levels of radical scavenging activities. However, the molecular mechanism underlying the color development and accumulation of different antioxidants in these rice cultivars remains largely elusive. Here, we report the proteome profiles of mature Super-hongmi, and Super-jami seeds and compared with the Hopum (white seeds) using a label-free quantitative proteomics approach. This approach led to the identification of 5127 rice seed proteins of which 1628 showed significant changes (ANOVA, Benjamini-Hochberg FDR ≤ 0.01, fold change ≥ 1.5). Functional annotation of the differentially modulated proteins led to the identification of a phytoene desaturase (PDS3) that was highly enriched in the red seeds and was decreased in the black seeds as compared to the control white seeds. PDS3 is involved in the conversion of phytoene to ζ-carotene which may be responsible for the accumulation of red color in red seeds. Moreover, black seeds seem to accumulate higher levels of anthocyanins because of the higher abundance of dihydroflavonol 4-reductase. In addition, proteins associated with lignin and tocopherol biosynthesis were found to be highly increased in both red and black cultivars. Taken together, these data report the seed proteome of three different colored rice seeds and identify novel components associated with pigment accumulation.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:03:35.171.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD039588
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSun Tae Kim
SpeciesList scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-20 03:35:07ID requested
12023-09-20 22:28:40announced
22023-11-14 09:05:26announced2023-11-14: Updated project metadata.
32024-10-22 06:03:36announced2024-10-22: Updated project metadata.
Publication List
10.1002/pmic.202300035;
Gupta R, Min CW, Jung JY, Ham TH, Jeon JS, Cho LH, Kwon SW, Kim ST, Proteome profiling highlights mechanisms underlying pigment and tocopherol accumulation in red and black rice seeds. Proteomics, 23(12):e2300035(2023) [pubmed]
10.6019/PXD039588;
Keyword List
submitter keyword: pigmented seeds, proteomics, seeds,Rice
Contact List
Sun Tae Kim
contact affiliationPusan National University, Miryang, South Korea
contact emailstkim5505@gmail.com
lab head
Sun Tae Kim
contact affiliationPusan National University
contact emailstkim5505@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD039588
PRIDE project URI
Repository Record List
[ + ]