PXD039586

PXD039586 is an original dataset announced via ProteomeXchange.

Title	MYC enhancer invasion promotes prognostic cancer type-specific gene programs through an epigenetic switch
Description	The transcription factor MYC is overexpressed in most cancers, where it drives multiple hallmarks of cancer progression. MYC is known to promote oncogenic transcription by binding to active promoters. In addition, MYC has also been shown to invade distal enhancers when expressed at oncogenic levels, but this enhancer binding has been proposed to have low gene-regulatory potential. Here, we demonstrate that MYC enhancer binding directly promotes cancer type-specific gene programs predictive of poor patient prognosis. MYC induces transcription of enhancer RNA through recruitment of RNAPII, rather than regulating RNAPII pause-release as is the case at promoters. This is mediated by MYC-induced H3K9 demethylation by KDM3A and acetylation by GCN5, leading to enhancer-specific BRD4 recruitment through its bromodomains, which facilitates RNAPII recruitment. Thus, we propose that MYC drives prognostic cancer type-specific gene programs by promoting RNAPII recruitment to enhancers through induction of an epigenetic switch.
HostingRepository	PRIDE
AnnounceDate	2023-11-30
AnnouncementXML	Submission_2023-11-30_06:11:19.542.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tina Ravnsborg
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-20 03:34:46	ID requested
⏵ 1	2023-11-30 06:11:19	announced

Dataset with its publication pending

submitter keyword: breast cancer, BRD4, Glucocorticoid receptor,MYC, GCN5, enhancer invasion, enhancer transcription, qplexRIME

Rasmus Siersbæk
contact affiliation	Department of Biochemistry and Molecular Biology, University of Southern Denmark
contact email	Siersbaek@bmb.sdu.dk
lab head
Tina Ravnsborg
contact affiliation	Department of Biochemisrty and Molecular Biology, University of Southern Denmark
contact email	tinaravnsborg@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD039586

PRIDE project URI

• PRIDE
  1. PXD039586
     1. Label: PRIDE project
     2. Name: MYC enhancer invasion promotes prognostic cancer type-specific gene programs through an epigenetic switch