PXD039553

PXD039553 is an original dataset announced via ProteomeXchange.

Title	Readout of histone methylation by Trim24 locally restricts chromatin opening by p53
Description	The genomic binding sites of the transcription factor (TF) and tumour suppressor p53 are unusually diverse in regards to their chromatin features, including histone modifications, opening the possibility that chromatin provides context-dependence for p53 regulation. Here, we show that the ability of p53 to open chromatin and activate its target genes is indeed locally restricted by its cofactor Trim24. Trim24 binds to both p53 and unmethylated lysine 4 of histone H3, thereby preferentially locating to those p53 sites that reside in closed chromatin, while it is deterred from accessible chromatin by lysine 4 methylation. The presence of Trim24 increases cell viability upon stress and enables p53 to impact gene expression as a function of the local chromatin state. These findings link histone methylation to p53 function and illustrate how specificity in chromatin can be achieved, not by TF-intrinsic sensitivity to histone modifications, but by employing chromatin-sensitive cofactors which locally modulate TF function.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:52.357.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Hess
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-18 16:30:05	ID requested
1	2023-05-05 02:40:18	announced
2	2023-07-03 03:48:39	announced	2023-07-03: Updated project metadata.
⏵ 3	2023-11-14 09:01:55	announced	2023-11-14: Updated project metadata.

Isbel L, Iskar M, Durdu S, Weiss J, Grand RS, Hietter-Pfeiffer E, Kozicka Z, Michael AK, Burger L, Thom, ä NH, Sch, ü, beler D, Readout of histone methylation by Trim24 locally restricts chromatin opening by p53. Nat Struct Mol Biol, 30(7):948-957(2023) [pubmed]

submitter keyword: Trim24 p53 mouse

Dr. Dirk Schuebeler
contact affiliation	Department of Gene Regulation Friedrich Miescher Institutefor Biomedical Research
contact email	dirk.schuebeler@fmi.ch
lab head
Daniel Hess
contact affiliation	Friedrich Miescher Institute for Biomedical Research
contact email	dhess@fmi.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD039553

PRIDE project URI

• PRIDE
  1. PXD039553
     1. Label: PRIDE project
     2. Name: Readout of histone methylation by Trim24 locally restricts chromatin opening by p53