PXD039539

PXD039539 is an original dataset announced via ProteomeXchange.

Title	Relative quantitation of coeliac toxic motifs and IgE epitopes in cereals containing gluten using proteomic profiling
Description	Cereals-based foods cause immune-mediated adverse reactions including coeliac disease and IgE-mediated allergies. Proteomic profiling of proteins carrying coeliac toxic motifs and allergens has been undertaken in wheat, barley, rye and oats in order to understand whether some cereal species, such as oats, maybe safe for allergic consumers. Total protein extracts were subject to chymotryptic digestion and analysed using data independent ion mobility mass spectrometry and analysed using a pipeline employing a curated gluten protein sequence database. Between 376-2769 proteins were identified and were dominated by proteins with a nutrient reservoir function. Relative quantitation of proteins containing coeliac toxic motifs showed they were present in wheat, barley and rye, but none were common to all three cereals, but were present in a limited number and much lower abundance in oats. The most abundant allergens were the seed storage prolamins, with allergens belonging to the α-amylase/trypsin inhibitor family associated with the inhalant allergy being of only moderate abundance. Wheat allergen homologues were identified in other cereal species but at a very low level in oats. These data suggest that the relative risk of oats in the context of both coeliac disease and IgE-mediated allergy is low.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-23_01:53:49.583.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew Daly
SpeciesList	scientific name: Secale cereale; NCBI TaxID: NEWT:4550; scientific name: Hordeum vulgare (Barley); NCBI TaxID: NEWT:4513; scientific name: Avena sativa; NCBI TaxID: NEWT:4498; scientific name: Triticum aestivum (Wheat); NCBI TaxID: NEWT:4565;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-18 07:33:24	ID requested
⏵ 1	2026-02-23 01:53:50	announced

10.1021/acs.jproteome.3c00456;

Daly ME, Huang X, Nitride C, Hughes C, Tanskanen J, Shewry PR, Gethings LA, Mills ENC, Proteomic Profiling of Celiac-Toxic Motifs and Allergens in Cereals Containing Gluten. J Proteome Res, 24(5):2336-2348(2025) [pubmed]

submitter keyword: coeliac disease, proteomic profiling,Gluten

E. N. Clare Mills
contact affiliation	Manchester Institute of Biotechnology, School of Biological Sciences, Manchester Academic Health Sciences Centre, The University of Manchester, Princess Street, Manchester, M1 7DN, UK
contact email	clare.mills@manchester.ac.uk
lab head
Matthew Daly
contact affiliation	University of Manchester
contact email	matthew.daly-3@postgrad.manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD039539

PRIDE project URI

• PRIDE
  1. PXD039539
     1. Label: PRIDE project
     2. Name: Relative quantitation of coeliac toxic motifs and IgE epitopes in cereals containing gluten using proteomic profiling