PXD039536

PXD039536 is an original dataset announced via ProteomeXchange.

Title	O-GlcNAcylation of Raptor transduces glucose signals to mTORC1
Description	Complex molecular mechanisms ensure cellular homeostasis between nutrient sensing and energy metabolism. Strong evidence from many laboratories supports a role for the hexosamine biosynthetic pathway (HBP) and its end product, UDP-GlcNAc, as important nutrient sensors. Isotopic photocleavable tagging for O-GlcNAc profiling (isoPTOP) was performed for quantitative and site specific identification of O-GlcNAcylation upon glucose mediated nutrient fluctuation. Mammalian target of Rapamycin complex 1（mTORC1）is a metabolic hub, which can sense the availability of various nutrients. We found that Raptor, the scaffold of the mTORC1 complex, is O-GlcNAcylated at T700. Our finding provides a novel mechanism that UDP-GlcNAc mediates glucose signal to mTORC1 by O-GlcNAcylating Raptor at T700 to regulate mTORC1 activity and cell growth.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:59:25.972.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoqing Pan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-18 07:31:43	ID requested
1	2023-08-03 08:00:00	announced
⏵ 2	2023-11-14 06:59:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: mTORC1, Raptor, isoPTOP, glucose,O-GlcNAc

Xing Chen
contact affiliation	College of Chemistry and Molecular Engineering, Peking University, Beijing, China
contact email	xingchen@pku.edu.cn
lab head
Xiaoqing Pan
contact affiliation	18856933419
contact email	xqpan@stu.pku.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD039536

PRIDE project URI

• PRIDE
  1. PXD039536
     1. Label: PRIDE project
     2. Name: O-GlcNAcylation of Raptor transduces glucose signals to mTORC1