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PXD039528

PXD039528 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleISAba1 mediated intrinsic chromosomal oxacillinase duplication conveys carbapenem resistance
DescriptionTandem duplication of carbapenemase genes amplifies the gene copy number and enhances carbapenem resistance. These amplifications are often heterogenous, transient, locate in plasmids, which often also contribute to heteroresistance. The duplication is especially important for low-hydrolysis activity enzymes, which is often overlooked or even under controversy. Here we reported an intrinsic oxacillinase duplication mediated by two neighbor ISAba1inserted in the same orientation. The duplication is relatively stable, located in chromosome, and the duplication times is up to twenty-five, much larger than previous reports. We provided genomic, transcriptomic, and proteomic evidence that the duplication resulted oxacillinase overproduction and thus contribute to carbapenem resistance. No other possible carbapenem resistance related changes (point mutations of oxaAb, disturbed expression of porin protein and efflux pump) were found during the duplication process. Furthermore, introducing oxaAb flanked by two ISAba1 to A. baumannii via plasmids, mimicked the in vivo duplication process under carbapenem stress. Taken together, ISAba1 mediated duplication of low activity intrinsic antibiotic hydrolysis enzymes could lead to antibiotic resistance for advanced-generation antibiotics.
HostingRepositoryiProX
AnnounceDate2023-01-17
AnnouncementXMLSubmission_2023-01-17_03:40:30.192.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLogen Liu
SpeciesList scientific name: Acinetobacter baumannii 470922; NCBI TaxID: 1310808;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-17 03:40:19ID requested
12023-01-17 03:40:30announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: A. baumannii, Carbapanem resistance
Contact List
Guoqing Li
contact affiliationThe Second Affiliated Hospital, University of South China
contact emailligq1970@163.com
lab head
Logen Liu
contact affiliationThe Second Affiliated Hospital, University of South China
contact emailllg@fsyy.usc.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI