PXD039528
PXD039528 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | ISAba1 mediated intrinsic chromosomal oxacillinase duplication conveys carbapenem resistance |
Description | Tandem duplication of carbapenemase genes amplifies the gene copy number and enhances carbapenem resistance. These amplifications are often heterogenous, transient, locate in plasmids, which often also contribute to heteroresistance. The duplication is especially important for low-hydrolysis activity enzymes, which is often overlooked or even under controversy. Here we reported an intrinsic oxacillinase duplication mediated by two neighbor ISAba1inserted in the same orientation. The duplication is relatively stable, located in chromosome, and the duplication times is up to twenty-five, much larger than previous reports. We provided genomic, transcriptomic, and proteomic evidence that the duplication resulted oxacillinase overproduction and thus contribute to carbapenem resistance. No other possible carbapenem resistance related changes (point mutations of oxaAb, disturbed expression of porin protein and efflux pump) were found during the duplication process. Furthermore, introducing oxaAb flanked by two ISAba1 to A. baumannii via plasmids, mimicked the in vivo duplication process under carbapenem stress. Taken together, ISAba1 mediated duplication of low activity intrinsic antibiotic hydrolysis enzymes could lead to antibiotic resistance for advanced-generation antibiotics. |
HostingRepository | iProX |
AnnounceDate | 2023-01-17 |
AnnouncementXML | Submission_2023-01-17_03:40:30.192.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Logen Liu |
SpeciesList | scientific name: Acinetobacter baumannii 470922; NCBI TaxID: 1310808; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-01-17 03:40:19 | ID requested | |
⏵ 1 | 2023-01-17 03:40:30 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: A. baumannii, Carbapanem resistance |
Contact List
Guoqing Li | |
---|---|
contact affiliation | The Second Affiliated Hospital, University of South China |
contact email | ligq1970@163.com |
lab head | |
Logen Liu | |
contact affiliation | The Second Affiliated Hospital, University of South China |
contact email | llg@fsyy.usc.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |