PXD039527

PXD039527 is an original dataset announced via ProteomeXchange.

Title	PekUSZ_Development of Lysine Crotonyl-mimic Probe to Covalently Identify H3K27Cr Interacting Proteins
Description	we developed a novel H3g27Cr peptide probe mimicking Histone lysine crotonylation, which could more effectively recognize Histone Kcr-interacting proteins through covalent proximity-induced bioconjugation. We initially validated the orthogonality and cysteine reactivity of crotonyl-like group through protein-ligand binding models of PDZΔRGS3 and PLCγ1-c SH2, and further showed the feasibility of H3g27Cr to covalently bind the known Kcr “eraser” HDAC1. With the H3g27Cr probe in hands, we successfully identified and validated one unknown H3K27Cr-interacting protein, the transcriptional activator STAT3, from AP-MS experiment in A549 cell lysates. The candidate protein STAT3 was found that directly bound to the Histone H3K27Ac/Cr in XL-MS and pull-down assays, and this interaction might reduce the H3K27Ac/Cr levels.
HostingRepository	iProX
AnnounceDate	2023-01-17
AnnouncementXML	Submission_2023-01-17_03:38:44.012.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuhao An
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-17 03:38:32	ID requested
⏵ 1	2023-01-17 03:38:44	announced

Dataset with its publication pending

submitter keyword: H3g27Cr, Histone, lysine crotonylation, STAT3, HDAC1, AP-MS

Rui Wang
contact affiliation	Pingshan Translational Medicine Center, Shenzhen Bay Laboratory
contact email	wangrui@szbl.ac.cn
lab head
Yuhao An
contact affiliation	Beijing Institute of Genomics, Chinese Academy of Sciences
contact email	anchiyuhao@vip.qq.com
dataset submitter

iProX dataset URI