PXD039521

PXD039521 is an original dataset announced via ProteomeXchange.

Title	Loss of H3K9 tri-methylation alters chromosome compaction and transcription factor retention during mitosis
Description	Recent studies have shown that repressive chromatin machinery, including DNA methyltransferases and Polycomb Repressor Complexes, bind to chromosomes throughout mitosis and their depletion results in increased chromosome size. Here we show that enzymes that catalyse H3K9 methylation, such as Suv39h1, Suv39h2, G9a and Glp, are also retained on mitotic chromosomes. Surprisingly however, mutants lacking H3K9me3 have unusually small and compact mitotic chromosomes associated with increased H3S10ph and H3K27me3 levels. Chromosome size and centromere compaction in these mutants were rescued by providing exogenous Suv39h1, or inhibiting Ezh2 activity. Quantitative proteomic comparisons of native mitotic chromosomes isolated from wildtype versus Suv39h1/Suv39h2 double-null mouse ESCs revealed that H3K9me3 was essential for the efficient retention of bookmarking factors such as Esrrb. These results highlight an unexpected role for repressive heterochromatin domains in preserving transcription factor binding through mitosis, and underscore the importance of H3K9me3 for sustaining chromosome architecture and epigenetic memory during cell division.
HostingRepository	PRIDE
AnnounceDate	2023-01-25
AnnouncementXML	Submission_2023-01-25_10:47:31.658.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AlexMontoya
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-17 01:30:46	ID requested
⏵ 1	2023-01-25 10:47:32	announced

Dataset with its publication pending

submitter keyword: Epigenetic memory - Mitotic chromosomes - H3K9me3 - Chromatin compaction - Heterochromatin - Mitotic bookmarking

Dr PavelShliaha
contact affiliation	MRC-LMS: Head of Proteomics and Metabolomics Core Facility
contact email	p.shliaha@lms.mrc.ac.uk
lab head
AlexMontoya
contact affiliation	Medical Research Council - London Institute of Medical Sciences
contact email	alex.montoya@lms.mrc.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039521
     1. Label: PRIDE project
     2. Name: Loss of H3K9 tri-methylation alters chromosome compaction and transcription factor retention during mitosis