PXD039481 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Probing antigen–antibody interactions using limited proteolysis–mass spectrometry |
Description | Protein–protein interactions (PPIs) mediate multiple essential functions and regulatory events in living organisms. The physical interactome of a given protein can be abnormally altered in response to external and internal cues, thus modulating cell physiology and contributing to human pathologies including neurodegenerative diseases. Despite substantial efforts, current approaches cannot pinpoint structure-specific PPIs or their interaction interfaces on a proteome-wide scale. To address this limitation, we have adapted the structural proteomics method known as limited proteolysis–mass spectrometry to identify PPIs by probing protein structural alterations within cellular extracts upon treatment with a protein of interest. We demonstrate the feasibility of our method by analysis of well-characterized PPIs between the respiratory syncytial virus F glycoprotein and its site-specific antibodies. Known antigenic sites were identified directly in complex biological matrices. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-10 |
AnnouncementXML | Submission_2024-02-10_15:54:08.308.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Aleš Holfeld |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse; Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-16 13:36:16 | ID requested | |
⏵ 1 | 2024-02-10 15:54:08 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LC-MSMS, Antibody-antigen interaction, Interactome, Mass spectrometry, Respiratory syncytial virus fusion glycoprotein,Limited proteolysis, Structural proteomics, Protein-protein interactions, Mammalian cells, Proteomics |
Contact List
Paola Picotti |
contact affiliation | Institute of Molecular Systems Biology, Department of Biology, ETH Zurich, Zurich, Switzerland |
contact email | picotti@imsb.biol.ethz.ch |
lab head | |
Aleš Holfeld |
contact affiliation | Institute of Molecular Systems Biology, Department of Biology, ETH Zurich, Zurich, Switzerland |
contact email | holfeld@imsb.biol.ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD039481 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039481
- Label: PRIDE project
- Name: Probing antigen–antibody interactions using limited proteolysis–mass spectrometry