PXD039468

PXD039468 is an original dataset announced via ProteomeXchange.

Title	MoaB2 binds to SigA in Mycobacterium smegmatis and modulates its activity and biological stability
Description	In mycobacteria, SigA is the primary sigma factor. This essential protein binds to RNA polymerase (RNAP) and is responsible for initiating transcription of housekeeping genes. Our knowledge about this factor in medicinally important mycobacteria is limited. Here, we performed an unbiased search for interacting partners of Mycobacterium smegmatis SigA. The search revealed a number of proteins; prominent among them was MoaB2. The SigA-MoaB2 interaction was validated and characterized by several approaches, revealing that it does not require RNAP and is highly specific, as alternative sigma factors did not interact with MoaB2. The structure of MoaB2 was solved by crystallography and the unique, unstructured N-terminal domain of SigA was identified to play a role in the SigA-MoaB2 interaction. Functional experiments showed that MoaB2 inhibits SigA-dependent transcription and increases biological stability of SigA. We propose that MoaB2, by sequestering SigA, exerts a dual effect on the transcription apparatus, and has the potential to modulate gene expression. In summary, this study has uncovered a new binding partner of mycobacterial SigA, paving the way for future research of this phenomenon.
HostingRepository	PRIDE
AnnounceDate	2024-11-11
AnnouncementXML	Submission_2024-11-11_06:11:22.639.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039468
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Martin Hubalek
SpeciesList	scientific name: Mycolicibacterium smegmatis MKD8; NCBI TaxID: 1214915;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-16 08:05:31	ID requested
⏵ 1	2024-11-11 06:11:23	announced

10.6019/PXD039468;

submitter keyword: Mycobacteria, transcription, RNA polymerase, SigA,MoaB2

Martin Hubálek
contact affiliation	Institute of Organic Chemistry and Biochemistry, CAS, Prague, Czech Republic
contact email	hubalek@uochb.cas.cz
lab head
Martin Hubalek
contact affiliation	Institute of Organic Chemistry and Biochemistry, Czech Academy of Science
contact email	hubalek@uochb.cas.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039468
     1. Label: PRIDE project
     2. Name: MoaB2 binds to SigA in Mycobacterium smegmatis and modulates its activity and biological stability