PXD039467 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of myelin basic protein interactome using TurboID proximity labeling proteomics |
Description | The protein environment of myelin basic protein (MBP), its uncharged form, and cyclindependent kinase inhibitor 1 (p21Cip1) were analyzed using two different approaches to identify protein partners thereof. The first one was a state-of-the-art enzymatic approach (TurboID), which utilizes an engineered biotin ligase that uses ATP to convert biotin to reactive biotin-AMP that covalently attaches to nearby proteins, followed by enrichment of biotin-labeled proteins on streptavidin beads and MS analysis of the resulting protein pool. Classical immunoprecipitation with Flag-tag fused to the target protein in combination with MS analysis was used as a reference method. As a result, two pools of potential interactors of MBP and uncharged MBP were identified. It was shown that TurboID, although giving a high level of background biotinylation, is a more reproducible technique compared to the immunoprecipitation, possibly due to higher affinity during the fraction enrichment process and easier maintaining of uniform conditions for the process, as well as interaction conditions being closer to in vivo conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2023-01-25 |
AnnouncementXML | Submission_2023-01-25_08:50:29.434.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD039467 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | GeorgijArapidi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-16 08:05:03 | ID requested | |
⏵ 1 | 2023-01-25 08:50:29 | announced | |
Publication List
Keyword List
submitter keyword: proximity labeling proteomics, multiple sclerosis, immunoprecipitation,myelin basic protein, membrane |
Contact List
GeorgijArapidi |
contact affiliation | System Biology Lab. at Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435, Russian Federation, Moscow, Malaya Pirogovskaya, 1a |
contact email | arapidi@gmail.com |
lab head | |
GeorgijArapidi |
contact affiliation | Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency |
contact email | arapidi@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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- PRIDE
- PXD039467
- Label: PRIDE project
- Name: Identification of myelin basic protein interactome using TurboID proximity labeling proteomics