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PXD039467

PXD039467 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of myelin basic protein interactome using TurboID proximity labeling proteomics
DescriptionThe protein environment of myelin basic protein (MBP), its uncharged form, and cyclindependent kinase inhibitor 1 (p21Cip1) were analyzed using two different approaches to identify protein partners thereof. The first one was a state-of-the-art enzymatic approach (TurboID), which utilizes an engineered biotin ligase that uses ATP to convert biotin to reactive biotin-AMP that covalently attaches to nearby proteins, followed by enrichment of biotin-labeled proteins on streptavidin beads and MS analysis of the resulting protein pool. Classical immunoprecipitation with Flag-tag fused to the target protein in combination with MS analysis was used as a reference method. As a result, two pools of potential interactors of MBP and uncharged MBP were identified. It was shown that TurboID, although giving a high level of background biotinylation, is a more reproducible technique compared to the immunoprecipitation, possibly due to higher affinity during the fraction enrichment process and easier maintaining of uniform conditions for the process, as well as interaction conditions being closer to in vivo conditions.
HostingRepositoryPRIDE
AnnounceDate2023-01-25
AnnouncementXMLSubmission_2023-01-25_08:50:29.434.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD039467
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterGeorgijArapidi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListbiotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-16 08:05:03ID requested
12023-01-25 08:50:29announced
Publication List
10.6019/PXD039467;
Keyword List
submitter keyword: proximity labeling proteomics, multiple sclerosis, immunoprecipitation,myelin basic protein, membrane
Contact List
GeorgijArapidi
contact affiliationSystem Biology Lab. at Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435, Russian Federation, Moscow, Malaya Pirogovskaya, 1a
contact emailarapidi@gmail.com
lab head
GeorgijArapidi
contact affiliationFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
contact emailarapidi@gmail.com
dataset submitter
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Dataset FTP location
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